Document Detail


Isolation and characterization of isolectins from Erythrina variegata seeds.
MedLine Citation:
PMID:  1517319     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Three isolectins were isolated from seeds of Erythrina variegata (Linn.) var. Orientalis by ion-exchange chromatography, followed by affinity chromatography on lactose-Sepharose 4B and acid-treated Sepharose 4B columns. The purified isolectins (EVLI, EVLII and EVLIII) are all specific for galactopyranosides and N-acetylgalactosamine, and their affinities for simple sugars are EVLIII greater than EVLII greater than EVLI. EVLI and EVLIII are homodimers made up of an A-subunit of molecular mass 36,000 and a B-subunit of molecular mass 33,000, whereas EVLII is a heterodimer composed of the A- and B-subunits. Upon treatment with trifluoromethansulphonic acid, the molecular masses of both subunits decreased to 31,000. Rechromatography of EVLII on the acid-treated Sepharose 4B column again produced the homodimeric lectins (EVLI and EVLIII). It is suggested that the constituent subunits of Erythrina variegata isolectins are eschangeable with each other in vitro.
Authors:
N Yamasaki; M Kimura; O Yamaguchi; M Araki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography     Volume:  597     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1992 Apr 
Date Detail:
Created Date:  1992-10-06     Completed Date:  1992-10-06     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  207-11     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Carbohydrates / analysis
Chromatography, Affinity
Chromatography, Gel
Chromatography, Ion Exchange
Hemagglutination
Lectins / isolation & purification*
Molecular Weight
Plant Lectins
Seeds / chemistry*
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Carbohydrates; 0/Lectins; 0/Plant Lectins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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