Document Detail

Isolation and characterization of isolectins from Erythrina variegata seeds.
MedLine Citation:
PMID:  1517319     Owner:  NLM     Status:  MEDLINE    
Three isolectins were isolated from seeds of Erythrina variegata (Linn.) var. Orientalis by ion-exchange chromatography, followed by affinity chromatography on lactose-Sepharose 4B and acid-treated Sepharose 4B columns. The purified isolectins (EVLI, EVLII and EVLIII) are all specific for galactopyranosides and N-acetylgalactosamine, and their affinities for simple sugars are EVLIII greater than EVLII greater than EVLI. EVLI and EVLIII are homodimers made up of an A-subunit of molecular mass 36,000 and a B-subunit of molecular mass 33,000, whereas EVLII is a heterodimer composed of the A- and B-subunits. Upon treatment with trifluoromethansulphonic acid, the molecular masses of both subunits decreased to 31,000. Rechromatography of EVLII on the acid-treated Sepharose 4B column again produced the homodimeric lectins (EVLI and EVLIII). It is suggested that the constituent subunits of Erythrina variegata isolectins are eschangeable with each other in vitro.
N Yamasaki; M Kimura; O Yamaguchi; M Araki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography     Volume:  597     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1992 Apr 
Date Detail:
Created Date:  1992-10-06     Completed Date:  1992-10-06     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  207-11     Citation Subset:  IM    
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MeSH Terms
Amino Acids / analysis
Carbohydrates / analysis
Chromatography, Affinity
Chromatography, Gel
Chromatography, Ion Exchange
Lectins / isolation & purification*
Molecular Weight
Plant Lectins
Seeds / chemistry*
Reg. No./Substance:
0/Amino Acids; 0/Carbohydrates; 0/Lectins; 0/Plant Lectins

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