Document Detail


Isolation, characterization and immunolocalization of phosphorylcholine-substituted glycolipids in developmental stages of Caenorhabditis elegans.
MedLine Citation:
PMID:  10583390     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Caenorhabditis elegans displays three neutral glycosphingolipids with structural homology to glycosphingolipids from the porcine nematode parasite, Ascaris suum. The present findings extend the degree of structural conservation between the two nematode species to glycosphingolipids with a phosphodiester substitution. Using a combination of hydrofluoric acid pretreatment, immunochemical characterization and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, three zwitterionic, phosphorylcholine-substituted glycosphingolipids could be identified in the neutral glycolipid fraction of C. elegans. The components were isolated as their zwitterionic, phosphorylcholine-substituted, pyridylaminated oligosaccharides by HPLC. Structural analysis was performed using hydrofluoric acid treatment, partial acid hydrolysis, methylation analysis, gas chromatography-mass spectrometry, cleavage with exoglycosidases and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Their chemical structures are proposed as: component Nz1, GalNAc(beta1-4)[phosphorylcholine]GlcNAc(beta1-3)Man(beta1-4)Glc-cera mide; component Nz2, Gal(alpha1-3)GalNAc(beta1-4)[phosphorylcholine]-GlcNAc(beta1-3)Man(be ta1-4)Glc-ceramide; and component Nz3, Gal(beta1-3)- Gal(alpha1-3)GalNAc(beta1-4)[phosphorylcholine]GlcNAc(beta1-3)Man(bet a1-4)Glc-ceramide. The oligosaccharide core is characteristic of the biosynthetic arthro-carbohydrate series of protostomial glycosphingolipids. The ceramide moiety was specified by a d17 : 1 sphingoid-base with iso-branching and anteiso-branching, and 2-hydroxy, saturated fatty acids as represented by docosanoic and tetracosanoic acids. Analysis of the spatial and temporal expression of the phosphorylcholine epitope, during embryonic and postembryonic development, showed it to be localized predominantly in seam cells and basement membranes, respectively. In early embryonic ontogenesis the phosphorylcholine epitope was only lipid bound, while in late embryonic and postembryonic development this epitope was both lipid bound and protein bound.
Authors:
S Gerdt; R D Dennis; G Borgonie; R Schnabel; R Geyer
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  266     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1999 Dec 
Date Detail:
Created Date:  2000-01-31     Completed Date:  2000-01-31     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  952-63     Citation Subset:  IM    
Affiliation:
Institute of Biochemistry, University of Giessen, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Caenorhabditis elegans / chemistry*,  growth & development,  immunology
Carbohydrate Sequence
Ceramides / chemistry
Chromatography, High Pressure Liquid
Epitopes / metabolism
Glycosphingolipids / immunology,  isolation & purification*,  metabolism
Immunohistochemistry
Molecular Sequence Data
Molecular Structure
Phosphorylcholine / chemistry*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Chemical
Reg. No./Substance:
0/Ceramides; 0/Epitopes; 0/Glycosphingolipids; 107-73-3/Phosphorylcholine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Extracellular calcium stimulates DNA synthesis in synergism with zinc, insulin and insulin-like grow...
Next Document:  Implications of hemolin glycosylation and Ca2+-binding on homophilic and cellular interactions.