Document Detail

Isolation and characterization of ichthyosan from tuna vitreous.
MedLine Citation:
PMID:  6221877     Owner:  NLM     Status:  MEDLINE    
Ichthyosan has been prepared from tuna vitreous. Glucuronic acid was found to account for the total uronic acid content of the macromolecule, while the hexosamine content was a mixture of N-acetyl-glucosamine and N-acetyl-galactosamine. When ichthyosan was gel filtered on Sepharose 2B or Sephacryl S-300, using sodium or calcium chloride, the elution profile of the column gave only one peak indicating no separation between glucosamine and galactosamine containing fractions. Similar results were obtained when ichthyosan was chromatographed on DEAE-cellulose using a salt gradient both in the presence and absence of 7.0 M urea. When ichthyosan was gel filtered in 4.0 M guanidine-HCL and subsequently chromatographed on DEAE-Sephacel or DEAE-cellulose, three well separated fractions were present. The two major fractions (II and III) were characterized as chondroitin and hyaluronic acid respectively; while fraction I representing about 3-5% of the total polysaccharide content of ichthyosan was identified as a keratan-like molecule. The same pattern was obtained when ichthyosan was digested with proteolytic enzymes and subsequently chromatographed on DEAE-cellulose or DEAE-Sephacel. Based on these findings it is concluded that in ichthyosan chondroitin, hyaluronate and keratan-like molecular chains are bound to proteins in non-covalent linkages.
G Armand; E A Balazs; K Meyer; M Reyes
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Connective tissue research     Volume:  11     ISSN:  0300-8207     ISO Abbreviation:  Connect. Tissue Res.     Publication Date:  1983  
Date Detail:
Created Date:  1983-07-08     Completed Date:  1983-07-08     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0365263     Medline TA:  Connect Tissue Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  21-33     Citation Subset:  IM    
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MeSH Terms
Chemical Fractionation
Chondroitin / analysis
Chromatography, DEAE-Cellulose
Chromatography, Gel
Hyaluronic Acid / analysis
Keratan Sulfate / analysis
Oligosaccharides / analysis
Polysaccharides / analysis,  isolation & purification*
Vitreous Body / analysis*
Grant Support
Reg. No./Substance:
0/Oligosaccharides; 0/Polysaccharides; 85568-45-2/ichythyosan; 9004-61-9/Hyaluronic Acid; 9007-27-6/Chondroitin; 9056-36-4/Keratan Sulfate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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