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Isolation and characterization of a human telomere.
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MedLine Citation:
PMID:  2549507     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method is described that allows cloning of human telomeres in S. cerevisiae by joining human telomeric restriction fragments to yeast artificial chromosome halves. The resulting chimeric yeast-human chromosomes propagate as true linear chromosomes, demonstrating that the human telomere structure is capable of functioning in yeast and suggesting that telomere functions are evolutionarily conserved between yeast and human. One cloned human telomere, yHT1, contains 4 kb of human genomic DNA sequence next to the tandemly repeating TTAGGG hexanucleotide. Genomic hybridizations using both cloned DNA and TTAGGG repeats have revealed a common structural organization of human telomeres. This 4 kb of genomic DNA sequence is present in most, but not all, human telomeres, suggesting that the region is not involved in crucial chromosome-specific functions. However, the extent of common features among the human telomeres and possible similarities in organization with yeast telomeres suggest that this region may play a role in general chromosome behavior such as telomere-telomere interactions. Unlike the simple telomeric TTAGGG repeats, our cloned human genomic DNA sequence does not cross-hybridize with rodent DNA. Thus, this clone allows the identifications of the terminal restriction fragments of specific human chromosomes in human-rodent hybrid cells.
Authors:
J F Cheng; C L Smith; C R Cantor
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Nucleic acids research     Volume:  17     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1989 Aug 
Date Detail:
Created Date:  1989-09-29     Completed Date:  1989-09-29     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  6109-27     Citation Subset:  IM    
Affiliation:
Department of Genetics, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Proteins*
Cell Line
Chromosome Mapping
Chromosomes, Fungal
Chromosomes, Human / ultrastructure*
Chromosomes, Human, Pair 21 / ultrastructure
Cloning, Molecular*
Cricetinae
DNA / genetics*
DNA Probes
DNA Restriction Enzymes
Deoxyribonuclease BamHI
Deoxyribonuclease EcoRI
Deoxyribonucleases, Type II Site-Specific
Genome, Human
Humans
Hybrid Cells
Nucleic Acid Hybridization
Repetitive Sequences, Nucleic Acid
Saccharomyces cerevisiae / genetics
Transformation, Genetic
Grant Support
ID/Acronym/Agency:
CA39782/CA/NCI NIH HHS; GM14825/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA Probes; 9007-49-2/DNA; EC 3.1.21.-/DNA Restriction Enzymes; EC 3.1.21.-/Deoxyribonuclease BamHI; EC 3.1.21.-/Deoxyribonuclease EcoRI; EC 3.1.21.4/BglII endonuclease; EC 3.1.21.4/Deoxyribonucleases, Type II Site-Specific; EC 3.1.21.4/GCGGCCGC-specific type II deoxyribonucleases
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
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Print publication date: Day: 11 Month: 8 Year: 1989
Volume: 17 Issue: 15
First Page: 6109 Last Page: 6127
ID: 318265
PubMed Id: 2549507

Isolation and characterization of a human telomere.
J F Cheng
C L Smith
C R Cantor
Department of Genetics, College of Physicians and Surgeons, Columbia University, New York, NY 10032.



Article Categories:
  • Research Article


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