Document Detail


Isolation and characterization of a human apoptosis-inducing gene with yeast two-hybrid system.
MedLine Citation:
PMID:  18726387     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
asy gene is a novel apoptosis-inducing gene, but its mechanism is unclear. To investigate the mechanism ofasy inducing apoptosis, a novel gene encoding ASY interacting protein (asyip) is isolated from human lung cell line (WI-38) cDNA library with yeast two-hybrid system. Theasyip gene is constitutively expressed as two mRNA transcripts with the size of 1.8 and 2.7 kb in various human tissues at different levels. Sequence analysis of full-length cDNA reveals that the two alternative transcripts ofasyip gene contain common 5' end and different 3' end, and share a common open reading frame encoding a polypeptide of 236 amino acids. Two protein kinase C phosphorylation sites and two casein kinase II phosphorylation sites are found in ASYIP amino acid sequence. Two highly hydrophobic regions encoding potentially two transmembrane domains are present. The ASYIP protein contains a C-terminal endoplasmic reticulum retrieval signal (Lys-Lys-Lys-Ala-Glu). Immunoprecipitation assay confirmed the interaction of ASY and ASYIP in mammalian cells. Compared withasy gene, overexpression ofasyip gene can inhibit growth of tumor cell Saos2 and induce cell apoptosis with a low efficiency.
Authors:
B Qi; Y Qi; Y Masuo; Q Liu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Science in China. Series C, Life sciences / Chinese Academy of Sciences     Volume:  43     ISSN:  1006-9305     ISO Abbreviation:  Sci. China, C, Life Sci.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2008-08-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9611809     Medline TA:  Sci China C Life Sci     Country:  China    
Other Details:
Languages:  eng     Pagination:  310-20     Citation Subset:  -    
Affiliation:
Institute of Virology, Wuhan University, 430072, Wuhan, China.
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