Document Detail


Isolation and characterization of hepatic stem cells, or "oval cells," from rat livers.
MedLine Citation:
PMID:  19089369     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The pace of research on the potential therapeutic uses of liver stem cells or "oval cells" has accelerated significantly in recent years. Concurrent advancements in techniques for the isolation and characterization of these cells have helped fuel this research. Several models now exist for the induction of oval cell proliferation in rodents. Protocols for the isolation and culture of these cells have evolved to the point that they may be set up in any laboratory equipped for cell culture. The advent of magnetic cell sorting has eliminated reliance on expensive flow cytometric sorting equipment to generate highly enriched populations of oval cells. Our laboratory has had much success in using the oval cell surface marker Thy-1 in combination with magnetic sorting to produce material suitable for testing the influence of a myriad of chemical signaling molecules on the oval cell phenotype. This chapter will describe our basic strategy for oval cell induction and isolation. Additionally, two in vitro procedures are described which the reader may find useful in the early stages of developing an oval cell research project.
Authors:
Thomas D Shupe; Anna C Piscaglia; Seh-Hoon Oh; Antonio Gasbarrini; Bryon E Petersen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  482     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2009  
Date Detail:
Created Date:  2008-12-17     Completed Date:  2009-02-11     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  387-405     Citation Subset:  IM    
Affiliation:
Department of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Movement
Cell Separation / methods*
Collagenases / metabolism
Flow Cytometry
Gravitation
Hepatectomy
Immunomagnetic Separation
Liver / cytology*
Perfusion
Rats
Stem Cells / cytology*
Grant Support
ID/Acronym/Agency:
R01 DK058614/DK/NIDDK NIH HHS; R01 DK058614-04/DK/NIDDK NIH HHS; R01 DK065096/DK/NIDDK NIH HHS; R01 DK065096-04/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
EC 3.4.24.-/Collagenases
Comments/Corrections

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