Document Detail


Isolation and characterization of a genomic DDD mouse interleukin-3 gene.
MedLine Citation:
PMID:  3935516     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A chromosomal DNA segment containing the entire gene for interleukin-3 (IL-3) was isolated from DDD mouse erythroleukemia cells, and its gene organization and nucleotide sequence were determined. Enhancer-like sequences in the second intron and G + C-rich sequence in the 5'-flanking region may play a role in the regulation of tissue-specific and inducible gene expression. Southern blot analyses revealed that constitutively IL-3-producing WEHI-3 cells have the rearranged IL-3 gene, and that genomic DNAs prepared from the adult and fetal mouse liver have the same organization of the IL-3 gene. No IL-3 gene transcript was detected in the mouse erythroleukemia cells by Northern blot analysis.
Authors:
K Todokoro; A Yamamoto; H Amanuma; Y Ikawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Gene     Volume:  39     ISSN:  0378-1119     ISO Abbreviation:  Gene     Publication Date:  1985  
Date Detail:
Created Date:  1986-02-19     Completed Date:  1986-02-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7706761     Medline TA:  Gene     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  103-7     Citation Subset:  IM    
Data Bank Information
Bank Name/Acc. No.:
GENBANK/M14394;  M20128
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Composition
Base Sequence
Cloning, Molecular
DNA / genetics
Genes*
Interleukin-3
Leukemia, Erythroblastic, Acute / genetics,  immunology
Lymphokines / genetics*
Mice
Chemical
Reg. No./Substance:
0/Interleukin-3; 0/Lymphokines; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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