Document Detail


Isolation and characterization of the gene encoding rat class theta glutathione S-transferase subunit yrs.
MedLine Citation:
PMID:  7802657     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The rat gene encoding the class theta glutathione S-transferase (GST) subunit Yrs has been isolated from a rat lambda EMBL4 DNA library and completely sequenced. The gene spans approximately 4 kb comprising 5 exons separated by 4 introns, the smallest number of the gene components among known rat genes encoding GST subunits. Sequence analysis of the 5' flanking region of the GST Yrs gene indicated the absence of both TATA and CAAT boxes. However, potential binding sites for the transcription factors SP1, PU1, PEA3, and AP-2 were present in this region. Primer extension and 5'-rapid amplification of cDNA ends studies demonstrated the existence of multiple transcription initiation sites distributed over 58 bp in the upstream sequence from the translation initiation codon. The present study provides the first evidence for the primary structure of the gene encoding a class theta GST subunit.
Authors:
K Ogura; T Nishiyama; A Hiratsuka; T Watabe; T Watabe
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  205     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1994 Dec 
Date Detail:
Created Date:  1995-01-25     Completed Date:  1995-01-25     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1250-6     Citation Subset:  IM    
Affiliation:
Department of Drug Metabolism and Molecular Toxicology, Tokyo College of Pharmacy, Japan.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/D38556
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Binding Sites
DNA / isolation & purification,  metabolism
DNA Primers
Exons
Gene Amplification
Genomic Library
Glutathione Transferase / biosynthesis,  genetics*
Humans
Introns
Isoenzymes / biosynthesis,  genetics*
Macromolecular Substances
Molecular Sequence Data
Promoter Regions, Genetic
Rats
Rats, Sprague-Dawley / genetics*
Repetitive Sequences, Nucleic Acid
Restriction Mapping
Sequence Homology, Amino Acid
TATA Box
Transcription Factors / metabolism
Chemical
Reg. No./Substance:
0/DNA Primers; 0/Isoenzymes; 0/Macromolecular Substances; 0/Transcription Factors; 9007-49-2/DNA; EC 2.5.1.-/Gstt2 protein, rat; EC 2.5.1.18/Glutathione Transferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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