| Isolation and characterization of four VIP-related peptides from red-bellied newt, Cynops pyrrhogaster. | |
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MedLine Citation:
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PMID: 15518909 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Four novel bioactive peptides were isolated from the red-bellied newt, Cynops pyrrhogaster, using a bioassay system monitoring the rectum contraction of the Japanese quail, Coturnix japonica. As these peptides are structurally related to vasoactive intestinal polypeptide (VIP), we termed these peptides newt VIP-related peptides 1, 2, 3, and 4 (NVRP-1, -2, -3, and -4). The primary sequences of these peptides were determined to be HSDAVFTDNYSRLLGKTALKNYLDGALKKE (NVRP-1), HSDAVFTDNYSRLLAKTALKNYLDGALKKE (NVRP-2), HSDAVFT-DNYSRLLGKIALKNYLDEALKKE (NVRP-3), and HSDAVFTDNYSRLLGKT-ALKNYLDSALKKE (NVRP-4). The N-terminal regions of these NVRPs possessed homology at the amino-acid level to various VIP, while the NVRP C-termini differed from VIPs significantly. All of the VIP consist of 28 amino-acid residues with amidated forms at the C-termini, whereas NVRPs possess 30 amino-acid residues and have free forms at the C-termini. NVRPs exert relaxant activities on isolated quail rectums in a dose-dependent manner, with threshold concentrations between 1 x 10(-8) and 3 x 10(-8) M. NVRPs also exhibited potent relaxant activities acting on the newt duodenum at 3 x 10(-8) M. As yet, this is the first isolation of biologically active VIP-related peptides from urodele. |
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Authors:
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Hitoshi Teranishi; Yojiro Muneoka; Toshifumi Takao; Yasutsugu Shimonishi; Masayasu Kojima |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Regulatory peptides Volume: 123 ISSN: 0167-0115 ISO Abbreviation: Regul. Pept. Publication Date: 2004 Dec |
Date Detail:
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Created Date: 2004-11-02 Completed Date: 2005-03-31 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8100479 Medline TA: Regul Pept Country: Netherlands |
Other Details:
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Languages: eng Pagination: 173-9 Citation Subset: IM |
Affiliation:
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Molecular Genetics, Institute of Life Science, Kurume University, Kurume, Fukuoka 839-0861, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Coturnix Duodenum / drug effects Female Gastrointestinal Hormones / chemistry, genetics, isolation & purification, pharmacology Male Molecular Sequence Data Muscle Relaxation / drug effects Peptide Hormones / chemistry, genetics, isolation & purification*, pharmacology Rectum / drug effects Salamandridae / genetics*, metabolism* Vasoactive Intestinal Peptide / chemistry, isolation & purification*, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Gastrointestinal Hormones; 0/Peptide Hormones; 37221-79-7/Vasoactive Intestinal Peptide |
| Comments/Corrections | |
Erratum In:
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Regul Pept. 2005 Jul 15;129(1-3):245 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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