Document Detail

Isolation and characterization of four VIP-related peptides from red-bellied newt, Cynops pyrrhogaster.
MedLine Citation:
PMID:  15518909     Owner:  NLM     Status:  MEDLINE    
Four novel bioactive peptides were isolated from the red-bellied newt, Cynops pyrrhogaster, using a bioassay system monitoring the rectum contraction of the Japanese quail, Coturnix japonica. As these peptides are structurally related to vasoactive intestinal polypeptide (VIP), we termed these peptides newt VIP-related peptides 1, 2, 3, and 4 (NVRP-1, -2, -3, and -4). The primary sequences of these peptides were determined to be HSDAVFTDNYSRLLGKTALKNYLDGALKKE (NVRP-1), HSDAVFTDNYSRLLAKTALKNYLDGALKKE (NVRP-2), HSDAVFT-DNYSRLLGKIALKNYLDEALKKE (NVRP-3), and HSDAVFTDNYSRLLGKT-ALKNYLDSALKKE (NVRP-4). The N-terminal regions of these NVRPs possessed homology at the amino-acid level to various VIP, while the NVRP C-termini differed from VIPs significantly. All of the VIP consist of 28 amino-acid residues with amidated forms at the C-termini, whereas NVRPs possess 30 amino-acid residues and have free forms at the C-termini. NVRPs exert relaxant activities on isolated quail rectums in a dose-dependent manner, with threshold concentrations between 1 x 10(-8) and 3 x 10(-8) M. NVRPs also exhibited potent relaxant activities acting on the newt duodenum at 3 x 10(-8) M. As yet, this is the first isolation of biologically active VIP-related peptides from urodele.
Hitoshi Teranishi; Yojiro Muneoka; Toshifumi Takao; Yasutsugu Shimonishi; Masayasu Kojima
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Regulatory peptides     Volume:  123     ISSN:  0167-0115     ISO Abbreviation:  Regul. Pept.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-11-02     Completed Date:  2005-03-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8100479     Medline TA:  Regul Pept     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  173-9     Citation Subset:  IM    
Molecular Genetics, Institute of Life Science, Kurume University, Kurume, Fukuoka 839-0861, Japan.
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MeSH Terms
Amino Acid Sequence
Duodenum / drug effects
Gastrointestinal Hormones / chemistry,  genetics,  isolation & purification,  pharmacology
Molecular Sequence Data
Muscle Relaxation / drug effects
Peptide Hormones / chemistry,  genetics,  isolation & purification*,  pharmacology
Rectum / drug effects
Salamandridae / genetics*,  metabolism*
Vasoactive Intestinal Peptide / chemistry,  isolation & purification*,  pharmacology
Reg. No./Substance:
0/Gastrointestinal Hormones; 0/Peptide Hormones; 37221-79-7/Vasoactive Intestinal Peptide
Erratum In:
Regul Pept. 2005 Jul 15;129(1-3):245

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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