Document Detail


Isolation and characterization of flat cells, a subpopulation of the embryonic chick retina.
MedLine Citation:
PMID:  6397876     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
When the embryonic neutral retina is dissociated into single cells which are maintained in stationary culture, the neuronal cells associate on the surfaces of a second population which we refer to as flat cells. The flat cells appear in the culture in significant numbers after 2 days and are required for neuronal cell attachment. We have been able to isolate pure flat cells from early cultures of mixed retina cells and have identified several antigens which support the concept that these cells are related to the glia. The cells have been tested by immunofluorescence for glial fibrillary acidic protein and have been found positive. Cell surfaces were labeled by transfer of tritiated galactose from UDP-galactose to endogenous acceptors in the presence of exogenous galactosyl transferase. After SDS-PAGE and fluorography, the surface glycoproteins of flat cells were seen to be significantly different from those of the original retina, and from chick fibroblasts. Immunoelectron microscope studies of detergent-extracted flat cells have demonstrated a complex network of intermediate filaments and actin fibers. We conclude that the flat cells are derived from the glia subpopulation of the retina and have adapted to the tissue culture environment by assuming this configuration. The unique surface properties of flat cells may be related to their role as an intermediate substrate between the neuronal cells and the tissue culture dish.
Authors:
H P Li; J B Sheffield
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Tissue & cell     Volume:  16     ISSN:  0040-8166     ISO Abbreviation:  Tissue Cell     Publication Date:  1984  
Date Detail:
Created Date:  1985-05-14     Completed Date:  1985-05-14     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0214745     Medline TA:  Tissue Cell     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  843-57     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Separation
Chick Embryo
Cytoplasm / ultrastructure
Cytoskeleton / ultrastructure
Glial Fibrillary Acidic Protein / analysis
Glycoproteins / analysis
Histocytochemistry
Immunologic Techniques
Intermediate Filament Proteins / analysis
Microscopy, Electron
Microtubules / ultrastructure
Retina / analysis,  cytology*,  embryology
Vimentin / analysis
Chemical
Reg. No./Substance:
0/Glial Fibrillary Acidic Protein; 0/Glycoproteins; 0/Intermediate Filament Proteins; 0/Vimentin

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