Document Detail


Isolation and characterization of fish scale collagen of higher thermal stability.
MedLine Citation:
PMID:  20116238     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Collagen is the most abundant protein found in animal body and widely used for biomedical and pharmaceutical applications. However, its applicability is severely limited due to high cost. Fish processing waste, which otherwise cause serious environmental pollution, is a promising cost effective collagen source. In the present study, collagen was isolated from scales of Labeo rohita (Rohu) and Catla catla (Catla). It is first time that these species are used as sources of collagen. Thermo-gravimetric analysis (TGA) revealed maximum demineralization achieved after 48h of EDTA treatment of intact scale. The isolated protein was confirmed as collagen by different physico-chemical techniques like FTIR, SDS-PAGE, and CD. Further amino acid analysis corroborates isolation of type I collagen. A major characteristic of obtained collagen was found to have denaturation temperature (T(d)) of 36.5 degrees C, which is promising as an advantage for biomedical application due to closeness in T(d) to mammalian collagen.
Authors:
Falguni Pati; Basudam Adhikari; Santanu Dhara
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Publication Detail:
Type:  Journal Article     Date:  2010-01-29
Journal Detail:
Title:  Bioresource technology     Volume:  101     ISSN:  1873-2976     ISO Abbreviation:  Bioresour. Technol.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-02-17     Completed Date:  2010-05-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9889523     Medline TA:  Bioresour Technol     Country:  England    
Other Details:
Languages:  eng     Pagination:  3737-42     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Ltd. All rights reserved.
Affiliation:
School of Medical Science and Technology, Indian Institute of Technology, Kharagpur 721302, India.
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MeSH Terms
Descriptor/Qualifier:
Animals
Circular Dichroism
Collagen / chemistry,  isolation & purification*
Electrophoresis, Polyacrylamide Gel
Fishes*
Hot Temperature
Spectroscopy, Fourier Transform Infrared
Chemical
Reg. No./Substance:
9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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