Document Detail

Isolation and characterization of a first set of polymorphic microsatellite markers in saffron, Crocus sativus (Iridaceae).
MedLine Citation:
PMID:  22935359     Owner:  NLM     Status:  In-Data-Review    
• Premise of the study: Twelve novel polymorphic microsatellite loci were developed and characterized from a repeat-enriched genomic library of Crocus sativus to study population and conservation genetics of this economically and medically important species. • Methods and Results: The microsatellite loci were isolated using a modified Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) method. The average number of alleles per locus was 2.6. The observed and expected heterozygosities varied from 0.07 to 0.92 and 0.1 to 0.58, respectively. Polymorphic information content value ranged from 0.09 to 0.55 with an average of 0.34. Four out of twelve loci showed significant departures from Hardy-Weinberg equilibrium. • Conclusions: The microsatellite markers reported here will be useful for evaluating genetic diversity and will likely serve as an ideal resource for use in marker-assisted breeding programs, germplasm analysis, and varietal identification.
Zahra Nemati; Mehrshad Zeinalabedini; Mohsen Mardi; Seyed M Pirseyediand; Seyed H Marashi; Seyed M Khayam Nekoui
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Publication Detail:
Type:  Journal Article     Date:  2012-08-30
Journal Detail:
Title:  American journal of botany     Volume:  99     ISSN:  1537-2197     ISO Abbreviation:  Am. J. Bot.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-09-07     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370467     Medline TA:  Am J Bot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e340-3     Citation Subset:  IM    
Department of Genomics, Agricultural Biotechnology Research Institute of Iran (ABRII), Seed and Plant Improvement Institutes Campus, Mahdasht Road, P.O. Box 31535-1897, Karaj, Iran.
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