Document Detail


Isolation and characterization of a psychropiezophilic alphaproteobacterium.
MedLine Citation:
PMID:  21948832     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cultivated psychropiezophilic (low-temperature- and high-pressure-adapted) bacteria are currently restricted to phylogenetically narrow groupings capable of growth under nutrient-replete conditions, limiting current knowledge of the extant functional attributes and evolutionary constraints of diverse microorganisms inhabiting the cold, deep ocean. This study documents the isolation of a deep-sea bacterium following dilution-to-extinction cultivation using a natural seawater medium at high hydrostatic pressure and low temperature. To our knowledge, this isolate, designated PRT1, is the slowest-growing (minimal doubling time, 36 h) and lowest cell density-producing (maximal densities of 5.0 × 10⁶ cells ml⁻¹) piezophile yet obtained. Optimal growth was at 80 MPa, correlating with the depth of capture (8,350 m), and 10°C, with average cell sizes of 1.46 μm in length and 0.59 μm in width. Through detailed growth studies, we provide further evidence for the temperature-pressure dependence of the growth rate for deep-ocean bacteria. PRT1 was phylogenetically placed within the Roseobacter clade, a bacterial lineage known for widespread geographic distribution and assorted lifestyle strategies in the marine environment. Additionally, the gene transfer agent (GTA) g5 capsid protein gene was amplified from PRT1, indicating a potential mechanism for increased genetic diversification through horizontal gene transfer within the hadopelagic environment. This study provides a phylogenetically novel isolate for future investigations of high-pressure adaptation, expands the known physiological traits of cultivated members of the Roseobacter lineage, and demonstrates the feasibility of cultivating novel microbial members from the deep ocean using natural seawater.
Authors:
Emiley A Eloe; Francesca Malfatti; Jennifer Gutierrez; Kevin Hardy; Wilford E Schmidt; Kit Pogliano; Joe Pogliano; Farooq Azam; Douglas H Bartlett
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-09-23
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  77     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-03     Completed Date:  2012-02-21     Revised Date:  2013-12-11    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8145-53     Citation Subset:  IM    
Data Bank Information
Bank Name/Acc. No.:
GENBANK/JF303756;  JF303757
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / genetics
Cluster Analysis
Cold Temperature
DNA, Bacterial / chemistry,  genetics
DNA, Ribosomal / chemistry,  genetics
Hydrostatic Pressure
Molecular Sequence Data
Phylogeny
RNA, Ribosomal, 16S / genetics
Roseobacter / classification*,  genetics,  growth & development,  isolation & purification*
Seawater / microbiology*
Sequence Analysis, DNA
Grant Support
ID/Acronym/Agency:
R01 GM057045/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA, Bacterial; 0/DNA, Ribosomal; 0/RNA, Ribosomal, 16S
Comments/Corrections

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