| Isolation and characterization of a psychropiezophilic alphaproteobacterium. | |
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MedLine Citation:
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PMID: 21948832 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cultivated psychropiezophilic (low-temperature- and high-pressure-adapted) bacteria are currently restricted to phylogenetically narrow groupings capable of growth under nutrient-replete conditions, limiting current knowledge of the extant functional attributes and evolutionary constraints of diverse microorganisms inhabiting the cold, deep ocean. This study documents the isolation of a deep-sea bacterium following dilution-to-extinction cultivation using a natural seawater medium at high hydrostatic pressure and low temperature. To our knowledge, this isolate, designated PRT1, is the slowest-growing (minimal doubling time, 36 h) and lowest cell density-producing (maximal densities of 5.0 × 10⁶ cells ml⁻¹) piezophile yet obtained. Optimal growth was at 80 MPa, correlating with the depth of capture (8,350 m), and 10°C, with average cell sizes of 1.46 μm in length and 0.59 μm in width. Through detailed growth studies, we provide further evidence for the temperature-pressure dependence of the growth rate for deep-ocean bacteria. PRT1 was phylogenetically placed within the Roseobacter clade, a bacterial lineage known for widespread geographic distribution and assorted lifestyle strategies in the marine environment. Additionally, the gene transfer agent (GTA) g5 capsid protein gene was amplified from PRT1, indicating a potential mechanism for increased genetic diversification through horizontal gene transfer within the hadopelagic environment. This study provides a phylogenetically novel isolate for future investigations of high-pressure adaptation, expands the known physiological traits of cultivated members of the Roseobacter lineage, and demonstrates the feasibility of cultivating novel microbial members from the deep ocean using natural seawater. |
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Authors:
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Emiley A Eloe; Francesca Malfatti; Jennifer Gutierrez; Kevin Hardy; Wilford E Schmidt; Kit Pogliano; Joe Pogliano; Farooq Azam; Douglas H Bartlett |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. Date: 2011-09-23 |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 77 ISSN: 1098-5336 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2011 Nov |
Date Detail:
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Created Date: 2011-11-03 Completed Date: 2012-02-21 Revised Date: 2012-05-01 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 8145-53 Citation Subset: IM |
Affiliation:
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Marine Biology Research Division, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, California 92093-0202, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/JF303756; JF303757 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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genetics Cluster Analysis Cold Temperature DNA, Bacterial / chemistry, genetics DNA, Ribosomal / chemistry, genetics Hydrostatic Pressure Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S / genetics Roseobacter / classification*, genetics, growth & development, isolation & purification* Seawater / microbiology* Sequence Analysis, DNA |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/DNA, Bacterial; 0/DNA, Ribosomal; 0/RNA, Ribosomal, 16S |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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