Document Detail

Isolation, characterization and expression of cationic peroxidase isozymes released into the medium of cultured tobacco cells.
MedLine Citation:
PMID:  7737186     Owner:  NLM     Status:  MEDLINE    
Three glycoproteins of 34, 38 and 40 kDa were isolated from the spent medium of suspension-cultured tobacco cells. The 38-kDa and 40-kDa proteins were highly cationic peroxidases with indistinguishable enzymic properties but their structural difference was confirmed by sequence analysis of the amino-terminal regions and the recognition specificity of monoclonal antibodies. The 34-kDa protein was a moderately cationic peroxidase with enzymic properties quite different from those of the 38-kDa and 40-kDa enzymes. They were undetectable in the spent medium during the cell-proliferation phase but became abundant in the medium during the cell-expansion phase. This was confirmed quantitatively with the 40-kDa protein using the 40-kDa-specific monoclonal antibody. The mRNA expression for 40-kDa protein was at a constant basal level in the cell-proliferation phase but increased in the cell-expansion phase.
H Narita; Y Asaka; K Ikura; S Matsumoto; R Sasaki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  228     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1995-06-02     Completed Date:  1995-06-02     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  855-62     Citation Subset:  IM    
Department of Food Science, Kyoto Women's University, Japan.
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MeSH Terms
Amino Acid Sequence
Antibodies, Monoclonal / immunology
Blotting, Northern
Cells, Cultured
Culture Media
Enzyme-Linked Immunosorbent Assay
Isoenzymes / genetics,  immunology,  isolation & purification*,  metabolism
Mice, Inbred BALB C
Molecular Sequence Data
Peroxidases / genetics,  immunology,  isolation & purification*,  metabolism
Plant Proteins / isolation & purification,  metabolism
Plants, Toxic*
Tobacco / enzymology*
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Culture Media; 0/Isoenzymes; 0/Plant Proteins; EC 1.11.1.-/Peroxidases

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