| Isolation, characterization and expression of cationic peroxidase isozymes released into the medium of cultured tobacco cells. | |
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MedLine Citation:
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PMID: 7737186 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Three glycoproteins of 34, 38 and 40 kDa were isolated from the spent medium of suspension-cultured tobacco cells. The 38-kDa and 40-kDa proteins were highly cationic peroxidases with indistinguishable enzymic properties but their structural difference was confirmed by sequence analysis of the amino-terminal regions and the recognition specificity of monoclonal antibodies. The 34-kDa protein was a moderately cationic peroxidase with enzymic properties quite different from those of the 38-kDa and 40-kDa enzymes. They were undetectable in the spent medium during the cell-proliferation phase but became abundant in the medium during the cell-expansion phase. This was confirmed quantitatively with the 40-kDa protein using the 40-kDa-specific monoclonal antibody. The mRNA expression for 40-kDa protein was at a constant basal level in the cell-proliferation phase but increased in the cell-expansion phase. |
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Authors:
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H Narita; Y Asaka; K Ikura; S Matsumoto; R Sasaki |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: European journal of biochemistry / FEBS Volume: 228 ISSN: 0014-2956 ISO Abbreviation: Eur. J. Biochem. Publication Date: 1995 Mar |
Date Detail:
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Created Date: 1995-06-02 Completed Date: 1995-06-02 Revised Date: 2007-07-23 |
Medline Journal Info:
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Nlm Unique ID: 0107600 Medline TA: Eur J Biochem Country: GERMANY |
Other Details:
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Languages: eng Pagination: 855-62 Citation Subset: IM |
Affiliation:
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Department of Food Science, Kyoto Women's University, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Antibodies, Monoclonal / immunology Blotting, Northern Cells, Cultured Culture Media Enzyme-Linked Immunosorbent Assay Female Isoenzymes / genetics, immunology, isolation & purification*, metabolism Mice Mice, Inbred BALB C Molecular Sequence Data Peroxidases / genetics, immunology, isolation & purification*, metabolism Plant Proteins / isolation & purification, metabolism Plants, Toxic* Tobacco / enzymology* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Culture Media; 0/Isoenzymes; 0/Plant Proteins; EC 1.11.1.-/Peroxidases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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