Document Detail

Isolation and characterization of the ecdysone receptor and its heterodimeric partner ultraspiracle through development in Sciara coprophila.
MedLine Citation:
PMID:  23321980     Owner:  NLM     Status:  MEDLINE    
Regulation of DNA replication is critical, and loss of control can lead to DNA amplification. Naturally occurring, developmentally regulated DNA amplification occurs in the DNA puffs of the late larval salivary gland giant polytene chromosomes in the fungus fly, Sciara coprophila. The steroid hormone ecdysone induces DNA amplification in Sciara, and the amplification origin of DNA puff II/9A contains a putative binding site for the ecdysone receptor (EcR). We report here the isolation, cloning, and characterizing of two ecdysone receptor isoforms in Sciara (ScEcR-A and ScEcR-B) and the heterodimeric partner, ultraspiracle (ScUSP). ScEcR-A is the predominant isoform in larval tissues and ScEcR-B in adult tissues, contrary to the pattern in Drosophila. Moreover, ScEcR-A is produced at amplification but is absent just prior. We discuss these results in relation to the model of ecdysone regulation of DNA amplification.
Michael S Foulk; John M Waggener; Janell M Johnson; Yutaka Yamamoto; Gerald M Liew; Fyodor D Urnov; Yuki Young; Genee Lee; Heidi S Smith; Susan A Gerbi
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-01-16
Journal Detail:
Title:  Chromosoma     Volume:  122     ISSN:  1432-0886     ISO Abbreviation:  Chromosoma     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-03-27     Completed Date:  2013-09-03     Revised Date:  2014-05-23    
Medline Journal Info:
Nlm Unique ID:  2985138R     Medline TA:  Chromosoma     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  103-19     Citation Subset:  IM    
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MeSH Terms
Amino Acid Sequence
Binding Sites
DNA Replication / genetics*
Diptera / cytology,  genetics*
Drosophila / cytology,  genetics
Ecdysone / genetics*
Larva / genetics,  metabolism
Protein Binding
Receptors, Steroid / genetics*,  isolation & purification,  metabolism
Salivary Glands / cytology
Grant Support
Reg. No./Substance:
0/Receptors, Steroid; 0/ecdysone receptor; 3604-87-3/Ecdysone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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