Document Detail


Isolation and characterization of distal lung progenitor cells.
MedLine Citation:
PMID:  22610556     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The majority of epithelial cells in the distal lung of rodents and humans are quiescent in vivo, yet certain cell populations retain an intrinsic capacity to proliferate and differentiate in response to lung injury or in appropriate culture settings, thus giving them properties of stem/progenitor cells. Here, we describe the isolation of two such populations from adult mouse lung: alveolar epithelial type 2 cells (AEC2), which can generate alveolar epithelial type 1 cells, and bronchioalveolar stem cells (BASCs), which in culture can reproduce themselves, as well as generate a small number of other distal lung epithelial cell types. These primary epithelial cells are typically isolated using enzyme digestion, mechanical disruption, and serial filtration. AEC2 and BASCs are distinguished from other distal lung cells by expression of specific markers as detected by fluorescence-activated cell sorting, immunohistochemistry, or a combination of both of these techniques.
Authors:
Barbara Driscoll; Alex Kikuchi; Allison N Lau; Jooeun Lee; Raghava Reddy; Edwin Jesudason; Carla F Kim; David Warburton
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  879     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2012-05-21     Completed Date:  2012-09-17     Revised Date:  2014-04-08    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  109-22     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Separation / methods*
Flow Cytometry
Immunohistochemistry
Lung / cytology*
Mice
Mice, Inbred Strains
Stem Cells / cytology*
Grant Support
ID/Acronym/Agency:
G0802057//Medical Research Council; R01 GM096195/GM/NIGMS NIH HHS; R01 HL065352/HL/NHLBI NIH HHS; R01 HL065352/HL/NHLBI NIH HHS; R01 HL090136/HL/NHLBI NIH HHS; R01 HL44060/HL/NHLBI NIH HHS
Comments/Corrections

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