| Isolation, characterization, and developmental expression of pig intestinal fatty acid-binding proteins. | |
| | |
MedLine Citation:
|
PMID: 15539179 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
|
The goal of this study was to characterize and quantify intestinal fatty acid-binding proteins of the pig. Small intestinal mucosa from 13-19 kg pigs was homogenized and centrifuged to obtain cytosol. Isolation of fatty acid-binding proteins from delipidated cytosol was achieved using molecular sieve, oleic acid affinity, and ion exchange chromatography. Fatty acid-binding protein isolation was monitored using a fatty-acid binding assay in conjunction with sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. Antisera to rat liver-fatty acid-binding protein cross reacted with an isolated intestinal fatty acid-binding protein of Mr = 13,000, whereas antisera to rat intestine-fatty acid-binding protein was not cross reactive with isolated pig intestinal proteins. These experiments identify a pig intestinal fatty acid-binding protein that exhibits strong immunochemical similarity to rat liver-fatty acid-binding protein. Cytosol prepared from intestinal mucosa of pigs at -4, 2, 4, 7, 15, 22, 28, and 35 d of age was assayed for fatty acid-binding protein activity. Preweaning fatty acid-binding protein activity in cytosol was maximal at 7 days of age when expressed as total jejunal fatty acid binding per kilogram bodyweight, intestinal or mucosal weight or milligram total protein. After weaning (21 d), fatty acid-binding protein activities declined to 28 days, but increased again by 35 days. Total soluble fatty acid-binding protein activity in pig intestine is regulated during postnatal development and this may account in part for the altered intestinal absorption of lipids observed in young pigs at weaning. |
| | |
Authors:
|
G A Reinhart; F A Simmen; D C Mahan; R C Simmen; M E White |
Related Documents
:
|
9546629 - Fatty acid-binding proteins in the heart. 11461829 - Ligand specificity and conformational stability of human fatty acid-binding proteins. 359539 - Removal by bovine serum albumin of fatty acids from membrane vesicles and its effect on... 2778809 - Leakage of heart fatty acid binding protein with ischemia and reperfusion in the rat. 1500069 - Monoclonal antibodies specific to sailfish serum albumin: development of an assay for t... 9770459 - The helical domain of intestinal fatty acid binding protein is critical for collisional... 15451309 - In vitro cytotoxic and genotoxic effects of diphenylarsinic acid, a degradation product... 8893759 - Contents and distributions of the proteoglycans decorin and biglycan in normal and oste... 6533029 - Destruction of carcinogenic and mutagenic n-nitrosamides in laboratory wastes. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: The Journal of nutritional biochemistry Volume: 1 ISSN: 0955-2863 ISO Abbreviation: J. Nutr. Biochem. Publication Date: 1990 Nov |
Date Detail:
|
Created Date: 2004-11-12 Completed Date: 2005-03-21 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9010081 Medline TA: J Nutr Biochem Country: United States |
Other Details:
|
Languages: eng Pagination: 592-8 Citation Subset: - |
Affiliation:
|
Department of Animal Science and Laboratories of Molecular and Developmental Biology, The Ohio State University, and The Ohio Agricultural Research and Development Center, Columbus, OH 43210-1095, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effects of dietary calcium on the metabolism of trace elements in male and female rats.
Next Document: Effects of iron deficiency upon the antibody response to influenza virus in rats.