Document Detail

Isolation, characterization, and developmental expression of pig intestinal fatty acid-binding proteins.
MedLine Citation:
PMID:  15539179     Owner:  NLM     Status:  PubMed-not-MEDLINE    
The goal of this study was to characterize and quantify intestinal fatty acid-binding proteins of the pig. Small intestinal mucosa from 13-19 kg pigs was homogenized and centrifuged to obtain cytosol. Isolation of fatty acid-binding proteins from delipidated cytosol was achieved using molecular sieve, oleic acid affinity, and ion exchange chromatography. Fatty acid-binding protein isolation was monitored using a fatty-acid binding assay in conjunction with sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. Antisera to rat liver-fatty acid-binding protein cross reacted with an isolated intestinal fatty acid-binding protein of Mr = 13,000, whereas antisera to rat intestine-fatty acid-binding protein was not cross reactive with isolated pig intestinal proteins. These experiments identify a pig intestinal fatty acid-binding protein that exhibits strong immunochemical similarity to rat liver-fatty acid-binding protein. Cytosol prepared from intestinal mucosa of pigs at -4, 2, 4, 7, 15, 22, 28, and 35 d of age was assayed for fatty acid-binding protein activity. Preweaning fatty acid-binding protein activity in cytosol was maximal at 7 days of age when expressed as total jejunal fatty acid binding per kilogram bodyweight, intestinal or mucosal weight or milligram total protein. After weaning (21 d), fatty acid-binding protein activities declined to 28 days, but increased again by 35 days. Total soluble fatty acid-binding protein activity in pig intestine is regulated during postnatal development and this may account in part for the altered intestinal absorption of lipids observed in young pigs at weaning.
G A Reinhart; F A Simmen; D C Mahan; R C Simmen; M E White
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of nutritional biochemistry     Volume:  1     ISSN:  0955-2863     ISO Abbreviation:  J. Nutr. Biochem.     Publication Date:  1990 Nov 
Date Detail:
Created Date:  2004-11-12     Completed Date:  2005-03-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9010081     Medline TA:  J Nutr Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  592-8     Citation Subset:  -    
Department of Animal Science and Laboratories of Molecular and Developmental Biology, The Ohio State University, and The Ohio Agricultural Research and Development Center, Columbus, OH 43210-1095, USA.
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