Document Detail


Isolation and characterization of cholic acid 7alpha-dehydroxylating fecal bacteria from cholesterol gallstone patients.
MedLine Citation:
PMID:  10673060     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND/AIMS: The development of cholesterol gallstones, in some patients, has been associated with increased proportions of deoxycholic acid in the bile acid pool. Deoxycholic acid is a microbial product of cholic acid 7alpha-dehydroxylation in the intestines. The levels and activities of bile acid 7alpha-dehydroxylating bacteria have been reported to be increased in gallstone patients. The aim of the current study was to isolate 7alpha-dehydroxylating bacteria from gallstone patients and determine if these individuals are colonized by similar bacterial species. METHODS: The levels of 7alpha-dehydroxylating bacteria in fecal samples were determined by fecal dilutions in 24 gallstone patients and 10 controls. 7alpha-Dehydroxylating bacteria were isolated by a non-selective streak plate technique and 7alpha-dehydroxylation activity was determined by measuring the conversion of [14C]-cholic acid to [14C]-deoxycholic acid using thin-layer chromatography. RESULTS: Gallstone patients had >42-fold (p<0.01) higher levels of 7alpha-dehydroxylating bacteria than patients who had not developed gallstones. Eighteen strains of 7alpha-dehydroxylating bacteria were isolated from eight gallstone patients. Attempts to isolate 7alpha-dehydroxylating bacteria from ten control patients were unsuccessful using identical isolation techniques. Surprisingly, all strains of bacteria isolated from gallstone patients appear to belong to the genus Clostridium. CONCLUSION: Gallstone patients have higher levels of 7alpha-dehydroxylating fecal bacteria and appear to harbor only members of the genus Clostridium with this activity.
Authors:
J E Wells; F Berr; L A Thomas; R H Dowling; P B Hylemon
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of hepatology     Volume:  32     ISSN:  0168-8278     ISO Abbreviation:  J. Hepatol.     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-02-24     Completed Date:  2000-02-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8503886     Medline TA:  J Hepatol     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  4-10     Citation Subset:  IM    
Affiliation:
Department of Microbiology and Immunology, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond 23298-0678, USA.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Bacterial Typing Techniques
Blotting, Southern
Cholelithiasis / microbiology*
Cholesterol*
Cholic Acid / metabolism
Cholic Acids / metabolism*
Chromatography, Thin Layer
Clostridium / genetics,  isolation & purification,  metabolism*
Colony Count, Microbial
DNA, Bacterial / analysis
Deoxycholic Acid / metabolism
Eubacterium / genetics,  isolation & purification,  metabolism*
Feces / microbiology*
Female
Humans
Male
Middle Aged
Steroid Hydroxylases / metabolism*
Grant Support
ID/Acronym/Agency:
F32DK-09750/DK/NIDDK NIH HHS; P01DK-38030/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Cholic Acids; 0/DNA, Bacterial; 57-88-5/Cholesterol; 81-25-4/Cholic Acid; 83-44-3/Deoxycholic Acid; EC 1.14.-/Steroid Hydroxylases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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