| Isolation and characterization of cholic acid 7alpha-dehydroxylating fecal bacteria from cholesterol gallstone patients. | |
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MedLine Citation:
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PMID: 10673060 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND/AIMS: The development of cholesterol gallstones, in some patients, has been associated with increased proportions of deoxycholic acid in the bile acid pool. Deoxycholic acid is a microbial product of cholic acid 7alpha-dehydroxylation in the intestines. The levels and activities of bile acid 7alpha-dehydroxylating bacteria have been reported to be increased in gallstone patients. The aim of the current study was to isolate 7alpha-dehydroxylating bacteria from gallstone patients and determine if these individuals are colonized by similar bacterial species. METHODS: The levels of 7alpha-dehydroxylating bacteria in fecal samples were determined by fecal dilutions in 24 gallstone patients and 10 controls. 7alpha-Dehydroxylating bacteria were isolated by a non-selective streak plate technique and 7alpha-dehydroxylation activity was determined by measuring the conversion of [14C]-cholic acid to [14C]-deoxycholic acid using thin-layer chromatography. RESULTS: Gallstone patients had >42-fold (p<0.01) higher levels of 7alpha-dehydroxylating bacteria than patients who had not developed gallstones. Eighteen strains of 7alpha-dehydroxylating bacteria were isolated from eight gallstone patients. Attempts to isolate 7alpha-dehydroxylating bacteria from ten control patients were unsuccessful using identical isolation techniques. Surprisingly, all strains of bacteria isolated from gallstone patients appear to belong to the genus Clostridium. CONCLUSION: Gallstone patients have higher levels of 7alpha-dehydroxylating fecal bacteria and appear to harbor only members of the genus Clostridium with this activity. |
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Authors:
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J E Wells; F Berr; L A Thomas; R H Dowling; P B Hylemon |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of hepatology Volume: 32 ISSN: 0168-8278 ISO Abbreviation: J. Hepatol. Publication Date: 2000 Jan |
Date Detail:
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Created Date: 2000-02-24 Completed Date: 2000-02-24 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8503886 Medline TA: J Hepatol Country: DENMARK |
Other Details:
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Languages: eng Pagination: 4-10 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Immunology, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond 23298-0678, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Bacterial Typing Techniques Blotting, Southern Cholelithiasis / microbiology* Cholesterol* Cholic Acid / metabolism Cholic Acids / metabolism* Chromatography, Thin Layer Clostridium / genetics, isolation & purification, metabolism* Colony Count, Microbial DNA, Bacterial / analysis Deoxycholic Acid / metabolism Eubacterium / genetics, isolation & purification, metabolism* Feces / microbiology* Female Humans Male Middle Aged Steroid Hydroxylases / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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F32DK-09750/DK/NIDDK NIH HHS; P01DK-38030/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cholic Acids; 0/DNA, Bacterial; 57-88-5/Cholesterol; 81-25-4/Cholic Acid; 83-44-3/Deoxycholic Acid; EC 1.14.-/Steroid Hydroxylases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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