Document Detail

Isolation and characterization of a chitinase cDNA from the entomopathogenic fungus, Metarhizium anisopliae.
MedLine Citation:
PMID:  9742698     Owner:  NLM     Status:  MEDLINE    
A novel chitinase gene was isolated from Metarhizium anisopliae grown in a medium containing chitin as the sole carbon source. Comparisons of nucleotide sequence of the isolated gene with those of other fungal chitinase genes showed low sequence identity (24.4-36.4%) except for the active site of chitinase. In addition, molecular mass determination of the fused gene product separated on a gel showed that the fused chitinase seems to be about 70 kDa, while the molecular mass calculated from the deduced amino acid sequence can be about 58 kDa. These molecular masses were different from values of 33 kDa for an endochitinase and 110 kDa for an exochitinase (N-acetylglucosaminidase) from M. anisopliae published previously.
S C Kang; S Park; D G Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  165     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  1998 Aug 
Date Detail:
Created Date:  1998-10-27     Completed Date:  1998-10-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  267-71     Citation Subset:  IM    
Department of Biotechnology, Taegu University, Kyungsan, South Korea.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Chitinase / genetics*,  metabolism
Cloning, Molecular
DNA, Complementary
DNA, Fungal
Genes, Fungal*
Mitosporic Fungi / enzymology,  genetics*
Molecular Sequence Data
Sequence Analysis, DNA
Reg. No./Substance:
0/DNA, Complementary; 0/DNA, Fungal; EC

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