Document Detail


Isolation and characterization of the cell-surface polysaccharides of Porphyromonas gingivalis ATCC 53978.
MedLine Citation:
PMID:  11154397     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cell-surface carbohydrates of Porphyromonas gingivalis strain ATCC 53978 were isolated and partially characterized. Three separate polysaccharides were found to be present: an extracellular polysaccharide, capsular polysaccharide and lipopolysaccharide. The capsular polysaccharide, which had peculiar, gel-like viscoelastic properties, was found to be comprised of mannuronic acid, glucuronic acid, galacturonic acid, galactose, and 2-acetamido-2-deoxy-D-glucose in relative molar ratios of 0.6:0.9:0.5:0.5:1.0, respectively. The extracellular polysaccharide was found to contain mannose, rhamnose, glucose, galactose, and 2-acetamido-2-deoxy-D-glucose in relative molar ratios of 13.5:1.4:1.0:2.0:1.0, respectively. The lipopolysaccharide was found to contain an O-antigen with a regular tetrasaccharide repeat unit comprised of 4-linked alpha-L-rhamnopyranosyl, 6-linked alpha-D-glucopyranosyl, 3-linked alpha-D-galactopyranosyl, and 4-linked 2-acetamido-2-deoxy-beta-D-glucopyranosyl residues in equimolar proportions.
Authors:
S I Farquharson; G R Germaine; G R Gray
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Oral microbiology and immunology     Volume:  15     ISSN:  0902-0055     ISO Abbreviation:  Oral Microbiol. Immunol.     Publication Date:  2000 Jun 
Date Detail:
Created Date:  2001-01-29     Completed Date:  2001-02-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8707451     Medline TA:  Oral Microbiol Immunol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  151-7     Citation Subset:  D    
Affiliation:
Department of Chemistry, University of Minnesota, 207 Pleasant St. SE, Minneapolis, MN 55455, USA.
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MeSH Terms
Descriptor/Qualifier:
Chromatography, Gas
Magnetic Resonance Spectroscopy
Osmolar Concentration
Polysaccharides, Bacterial / chemistry*,  isolation & purification*
Porphyromonas gingivalis / chemistry*
Grant Support
ID/Acronym/Agency:
GM 34710/GM/NIGMS NIH HHS; P50 DE08489/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Polysaccharides, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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