Document Detail


Isolation and characterization of cancer stem cells from a human glioblastoma cell line U87.
MedLine Citation:
PMID:  18343028     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A variety of malignant cancers have been found to contain a subpopulation of stem cell-like tumor cells, or cancer stem cells (CSCs). However, the existence of CSCs in U87, a most commonly used glioma cell line, is still controversial. In this study, we demonstrate that U87 cell line contained a fraction of tumor cells that could form tumor spheres and were enriched by progressively increasing the concentration of serum-free neural stem cell medium with or without low dose vincristine. These cells possessed the ability of self-renewal and multipotency, the defined characteristics of CSCs. Moreover, the tumors formed by the secondary spheres displayed typical histological features of human glioblastoma, including cellular pleomorphism, pseudopalisades surrounding necrosis, hyperchromatic nuclei, high density of microvessels and invasion to the brain parenchyma. These results indicate that gradually increasing the concentration of serum-free neural stem cell culture medium with or without vincristine is a simple and effective method for isolation of CSCs to study the initiation and progression of human glioblastoma.
Authors:
Shi-Cang Yu; Yi-Fang Ping; Liang Yi; Zhi-Hua Zhou; Jian-Hong Chen; Xiao-Hong Yao; Lei Gao; Ji Ming Wang; Xiu-Wu Bian
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-14
Journal Detail:
Title:  Cancer letters     Volume:  265     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-19     Completed Date:  2008-09-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  124-34     Citation Subset:  IM    
Affiliation:
Institute of Pathology, Southwest Hospital, Third Military Medical University, Gaotanyan 30, Shapingba District, Chongqing 400038, PR China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain Neoplasms / blood supply,  pathology*
Cell Differentiation
Cell Line, Tumor
Cell Proliferation
Cell Separation
Female
Glioblastoma / blood supply,  pathology*
Humans
Mice
Mice, Nude
Multidrug Resistance-Associated Proteins / biosynthesis,  genetics
Neoplastic Stem Cells / pathology*
RNA, Messenger / biosynthesis
Spheroids, Cellular / pathology*
Transplantation, Heterologous
Vincristine / pharmacology
Chemical
Reg. No./Substance:
0/Multidrug Resistance-Associated Proteins; 0/RNA, Messenger; 0/multidrug resistance-associated protein 1; 57-22-7/Vincristine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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