Document Detail

Isolation and characterization of a cDNA encoding a putative high mobility group (HMG)--box protein from stored mRNA in resting cysts of the ciliate Oxytricha (Sterkiella) nova: ciliate macronuclear gene encoding a putative HMG-box protein.
MedLine Citation:
PMID:  14672407     Owner:  NLM     Status:  MEDLINE    
We have isolated an cDNA after applying a DDRT-PCR analysis on mRNA from mature resting cysts of the ciliate Oxytricha (Sterkiella) nova. From this cDNA fragment the complete macronuclear minichromosome was obtained by using the Mac-End-PCR method. After cloning and sequencing, this cDNA shown certain similarity to HMG-like proteins. The analysis of the inferred amino acid sequence shown that this putative HMG-like protein has one HMG-box interrupted by a intron. The analysis of others characteristics (including a 3D model) confirms that it is a HMGB family protein. It is the first time that a macronuclear gene encoding a putative HMG-box protein is isolated from resting cysts of a stichotrich ciliate. The possible implications of this stored mRNA in the ciliate cryptobiotic stage are discussed.
Sergio Callejas; Juan Carlos Gutiérrez
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular biology reports     Volume:  30     ISSN:  0301-4851     ISO Abbreviation:  Mol. Biol. Rep.     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2003-12-15     Completed Date:  2004-07-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0403234     Medline TA:  Mol Biol Rep     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  215-22     Citation Subset:  IM    
Departamento de Microbiología-111, Facultad de Biología, C/. José Antonio Novais, 2, Universidad Complutense (UCM), 28040 Madrid, Spain.
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MeSH Terms
Amino Acid Sequence
Base Sequence
DNA, Complementary / genetics*,  isolation & purification*,  metabolism
HMGB Proteins / chemistry,  genetics*
Models, Molecular
Molecular Sequence Data
Oxytricha / cytology,  genetics,  metabolism*
RNA, Messenger, Stored / genetics*,  isolation & purification,  metabolism
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Reg. No./Substance:
0/DNA, Complementary; 0/HMGB Proteins; 0/RNA, Messenger, Stored

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