| Isolation, characterization and biological evaluation of bioactive metabolites from Nocardia levis MK-VL_113. | |
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MedLine Citation:
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PMID: 19577444 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An Actinomycete isolate found to be prominent in the laterite soils of Acharya Nagarjuna University (ANU) Campus, Guntur was identified as Nocardia levis MK-VL_113 by 16S rRNA analysis. Cultural, morphological and physiological characteristics of the strain were recorded. Screening of secondary metabolites obtained from 4-day old culture broth of the strain led to the isolation of two fractions active against a wide variety of Gram-positive, Gram-negative bacteria and fungi. The structure of the first active fraction was elucidated using FT-IR, EI-MS, (1)H NMR and (13)C NMR spectra and identified as 1-phenylbut-3-ene-2-ol which is first time reported as a natural product. The compound exhibited good antimicrobial potential against the opportunistic and pathogenic bacteria and fungi. The antifungal activity of the strain and its metabolite were further confirmed with in vitro and in vivo studies. Evidence for the antagonism of the strain against Fusarium oxysporum, causing wilt disease in sorghum was demonstrated by the formation of inhibition zone in in vitro plate assay and reduction in the incidence of wilt of sorghum plants by using a green house trial. Analysis of the rhizosphere soil extracts by high performance liquid chromatography also demonstrated the production of the compound by the strain under in vivo conditions. As compared to the commercial fungicide mancozeb, the bioactive compound, 1-phenylbut-3-ene-2-ol was highly effective in controlling wilt of sorghum. Besides, the partially purified second fraction (PPF) subjected to gas chromatography-mass spectrometry revealed the presence of phenylethyl alcohol, dibutyl phthalate and 1,2-benzenedicarboxylic acid, 3-nitro. |
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Authors:
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Alapati Kavitha; Peddikotla Prabhakar; Manchala Narasimhulu; Muvva Vijayalakshmi; Yenamandra Venkateswarlu; Karanam Venkateswara Rao; Venkata Balaraju Subba Raju |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-07-03 |
Journal Detail:
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Title: Microbiological research Volume: 165 ISSN: 1618-0623 ISO Abbreviation: Microbiol. Res. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-03-08 Completed Date: 2010-05-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9437794 Medline TA: Microbiol Res Country: Germany |
Other Details:
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Languages: eng Pagination: 199-210 Citation Subset: IM |
Affiliation:
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Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur 522 510, India. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/FJ209734 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anti-Bacterial Agents
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chemistry,
isolation & purification,
pharmacology Antifungal Agents / chemistry, isolation & purification, pharmacology Biological Products / isolation & purification*, pharmacology* DNA, Bacterial / chemistry, genetics DNA, Ribosomal / chemistry, genetics Fusarium / drug effects India Microbial Sensitivity Tests Molecular Sequence Data Nocardia / chemistry*, classification, genetics, isolation & purification* Plant Diseases / microbiology RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Soil Microbiology Sorghum / microbiology Spectrum Analysis |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Antifungal Agents; 0/Biological Products; 0/DNA, Bacterial; 0/DNA, Ribosomal; 0/RNA, Ribosomal, 16S |
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