Document Detail


Isolation and characterization of basal cells from human upper respiratory epithelium.
MedLine Citation:
PMID:  9434631     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cellular pathways of normal and reparative differentiation of upper airway epithelium are not well understood. Of the three main cell types, basal and secretory cells are known to divide, while ciliated cells are considered terminally differentiated. Several investigations support the role of the basal cell as a progenitor cell type, but others suggest that the secretory cell can regenerate a complete mucocilliary epithelium. Thus, lineage relationships within renewing adult epithelia are still unclear. Understanding the pathways involved in upper airway epithelial cell differentiation is critical for studying injury and repair mechanisms and for developing clinical strategies for tracheal reconstruction. We undertook the current studies to determine the integrin profile of isolated human upper airway basal cells. Respiratory epithelial cells (REC) were isolated by elastase digestion, stained with FITC-labeled Griffonia simplicifolia isolectin B4 (GSI-B4), and sorted by flow cytometry. Approximately 80% of the lectin-positive cells were basal cells, as determined by morphology and cytokeratin staining. These cells expressed integrins alpha 1, alpha 2, alpha 3, alpha 5, alpha v beta 5, beta 1, beta 3, and alpha 6 beta 4, by immunohistochemistry. This is the first report to identify the integrin profile of isolated human upper airway basal cells. These basal cells could be maintained on type I collagen for at least 7 days, where they became partially confluent and retained expression of cytokeratins 5 and 14. Availability of pure populations of basal cells should permit investigations of their role in both normal and maladaptive repair of adult upper airway epithelium.
Authors:
W Hicks; L Hall; L Sigurdson; C Stewart; R Hard; J Winston; J Lwebuga-Mukasa
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Experimental cell research     Volume:  237     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1997 Dec 
Date Detail:
Created Date:  1998-02-03     Completed Date:  1998-02-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  357-63     Citation Subset:  IM    
Affiliation:
Department of Head and Neck Surgery, Roswell Park Cancer Institute, Buffalo, New York 14263, USA.
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MeSH Terms
Descriptor/Qualifier:
Adult
Cell Separation
Cells, Cultured
Epithelial Cells / cytology*
Humans
Integrins / metabolism
Keratins / metabolism
Lectins / diagnostic use
Nasal Polyps / pathology*
Respiratory System / cytology*
Time Factors
Chemical
Reg. No./Substance:
0/Integrins; 0/Lectins; 68238-35-7/Keratins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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