Document Detail


Isolation and characterization of bacteria capable of degrading polycyclic aromatic hydrocarbons (PAHs) and organophosphorus pesticides from PAH-contaminated soil in Hilo, Hawaii.
MedLine Citation:
PMID:  17552538     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nineteen bacterial strains were isolated from petroleum-contaminated soil in Hilo, HI, and characterized by two different spray-plated methods, turbidity test in liquid medium, and 16S rRNA gene sequence analysis. Analysis of the soil showed 13 polycyclic aromatic hydrocarbons (PAHs) in a range from 0.6 to 30 mg/kg of dry weight each and 12 PAH metabolites. Five distinct bacterial strains (C3, C4, P1-1, JS14, and JS19b1) selected from preliminary plating and turbidity tests were further tested for PAH degradation through single PAH degradation assay. Strains C3, C4, and P1-1 degraded phenanthrene (40 mg/L) completely during 7 days of incubation. Strain JS14 degraded fluoranthene (40 mg/L) completely during 10 days of incubation. Strain JS19b1 degraded 100% of phenanthrene (40 mg/L) in 7 days, 77% of fluorene (40 mg/L) in 14 days, 97% of fluoranthene (40 mg/L) in 10 days, and 100% of pyrene (40 mg/L) in 14 days. Turbidity tests showed that strains P1-1, JS14, and JS19b1 utilized several organophosphorus pesticides as growth substrate. P1-1 can degrade carbofenothion, chlorfenvinphos, diazinon, fonofos, and pirimiphos-methyl. JS14 can transform chlorfenvinphos and diazinon. JS19b1 can break down diazinon, pirimiphos-methyl, and temephos.
Authors:
Jong-Su Seo; Young-Soo Keum; Renee M Harada; Qing X Li
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2007-06-07
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  55     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-07-03     Completed Date:  2007-09-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5383-9     Citation Subset:  IM    
Affiliation:
Department of Molecular Biosciences and Bioengineering, University of Hawaii, 1955 East-West Road, Honolulu, Hawaii 96822, USA.
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MeSH Terms
Descriptor/Qualifier:
Bacteria / genetics,  isolation & purification*,  metabolism*
DNA, Bacterial / chemistry
Hawaii
Organophosphorus Compounds / metabolism*
Pesticides / metabolism*
Polycyclic Hydrocarbons, Aromatic / analysis,  metabolism*
RNA, Ribosomal, 16S / genetics
Soil / analysis
Soil Microbiology*
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/Organophosphorus Compounds; 0/Pesticides; 0/Polycyclic Hydrocarbons, Aromatic; 0/RNA, Ribosomal, 16S; 0/Soil

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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