| Isolation and characterization of bacteria capable of degrading polycyclic aromatic hydrocarbons (PAHs) and organophosphorus pesticides from PAH-contaminated soil in Hilo, Hawaii. | |
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MedLine Citation:
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PMID: 17552538 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nineteen bacterial strains were isolated from petroleum-contaminated soil in Hilo, HI, and characterized by two different spray-plated methods, turbidity test in liquid medium, and 16S rRNA gene sequence analysis. Analysis of the soil showed 13 polycyclic aromatic hydrocarbons (PAHs) in a range from 0.6 to 30 mg/kg of dry weight each and 12 PAH metabolites. Five distinct bacterial strains (C3, C4, P1-1, JS14, and JS19b1) selected from preliminary plating and turbidity tests were further tested for PAH degradation through single PAH degradation assay. Strains C3, C4, and P1-1 degraded phenanthrene (40 mg/L) completely during 7 days of incubation. Strain JS14 degraded fluoranthene (40 mg/L) completely during 10 days of incubation. Strain JS19b1 degraded 100% of phenanthrene (40 mg/L) in 7 days, 77% of fluorene (40 mg/L) in 14 days, 97% of fluoranthene (40 mg/L) in 10 days, and 100% of pyrene (40 mg/L) in 14 days. Turbidity tests showed that strains P1-1, JS14, and JS19b1 utilized several organophosphorus pesticides as growth substrate. P1-1 can degrade carbofenothion, chlorfenvinphos, diazinon, fonofos, and pirimiphos-methyl. JS14 can transform chlorfenvinphos and diazinon. JS19b1 can break down diazinon, pirimiphos-methyl, and temephos. |
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Authors:
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Jong-Su Seo; Young-Soo Keum; Renee M Harada; Qing X Li |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. Date: 2007-06-07 |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 55 ISSN: 0021-8561 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2007 Jul |
Date Detail:
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Created Date: 2007-07-03 Completed Date: 2007-09-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 5383-9 Citation Subset: IM |
Affiliation:
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Department of Molecular Biosciences and Bioengineering, University of Hawaii, 1955 East-West Road, Honolulu, Hawaii 96822, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacteria
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genetics,
isolation & purification*,
metabolism* DNA, Bacterial / chemistry Hawaii Organophosphorus Compounds / metabolism* Pesticides / metabolism* Polycyclic Hydrocarbons, Aromatic / analysis, metabolism* RNA, Ribosomal, 16S / genetics Soil / analysis Soil Microbiology* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/Organophosphorus Compounds; 0/Pesticides; 0/Polycyclic Hydrocarbons, Aromatic; 0/RNA, Ribosomal, 16S; 0/Soil |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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