Document Detail

Isolation and characterization of an amiloride-resistant mutant of Methanothermobacter thermautotrophicus possessing a defective Na+/H+ antiport.
MedLine Citation:
PMID:  17286571     Owner:  NLM     Status:  MEDLINE    
A spontaneous mutant of Methanothermobacter thermautotrophicus resistant to the Na+/H+ antiporter inhibitor amiloride was isolated. The Na+/H+ exchanger activity in the mutant cells was remarkably decreased in comparison with wild-type cells. Methanogenesis rates in the mutant strain were higher than wild-type cells and resistant to the inhibitory effect of 2 mM amiloride. In contrast, methanogenesis in wild-type cells was completely inhibited by the same amiloride concentration. ATP synthesis driven by methanogenic electron transport or by an electrogenic potassium efflux in the presence of sodium ions was significantly enhanced in the mutant cells. ATP synthesis driven by potassium diffusion potential was profoundly inhibited in wild-type cells by the presence of uncoupler 3,3',4',5- tetrachlorosalicylanilide and sodium ions, whereas c. 50% inhibition was observed in the mutant cells under the same conditions.
Stanislav Surín; L'ubomíra Cubonová; Alan I Majerník; Paul McDermott; James P J Chong; Peter Smigán
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-02-05
Journal Detail:
Title:  FEMS microbiology letters     Volume:  269     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-03-29     Completed Date:  2007-06-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  England    
Other Details:
Languages:  eng     Pagination:  301-8     Citation Subset:  IM    
Institute of Animal Biochemistry and Genetics, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovakia.
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MeSH Terms
Adenosine Triphosphate / metabolism
Amiloride / pharmacology*
Drug Resistance, Bacterial*
Methane / metabolism
Methanobacteriaceae / classification*,  drug effects,  genetics,  isolation & purification
Sodium Channel Blockers / pharmacology*
Sodium-Hydrogen Antiporter / antagonists & inhibitors*
Reg. No./Substance:
0/Sodium Channel Blockers; 0/Sodium-Hydrogen Antiporter; 2609-46-3/Amiloride; 56-65-5/Adenosine Triphosphate; 74-82-8/Methane

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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