Document Detail


Isolation and characterization of ZH14 with antiviral activity against Tobacco mosaic virus.
MedLine Citation:
PMID:  18535629     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A large number of bacteria were isolated from plant samples and screened for antiviral activity against the Tobacco mosaic virus (TMV). The bacterium ZH14, which was isolated from Chinese Anxi oolong tea, secreted the antiviral substances, having 94.2% virus inhibition when the bacterial culture filtrate and TMV extract were mixed at a ratio of 1:1. The ZH14 strain is a gram-positive, spore-forming rod and has the ability to degrade ribonucleic acid. Based on its effectiveness on virus inhibition, ZH14 was selected for characterization and was identified as a strain of the Bacillus cereus group based on phenotypic tests and comparative analysis of its 16S rDNA sequence. At the same time, we determined the antiviral product of ZH14 as an extracellular protein with high molecular mass, having an optimum temperature of 15-60 degrees C and an optimum pH of 6-10. Hence, the ZH14 strain and its culture filtrate have potential application in controlling plant diseases caused by TMV.
Authors:
Wen-Wen Zhou; Li-Xiang Zhang; Bin Zhang; Fei Wang; Zhi-Hong Liang; Tian-Gui Niu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Canadian journal of microbiology     Volume:  54     ISSN:  0008-4166     ISO Abbreviation:  Can. J. Microbiol.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-06-06     Completed Date:  2008-09-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372707     Medline TA:  Can J Microbiol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  441-9     Citation Subset:  IM    
Affiliation:
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China. zww2003@gmail.com
Data Bank Information
Bank Name/Acc. No.:
GENBANK/EF468497
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MeSH Terms
Descriptor/Qualifier:
Antiviral Agents / chemistry*,  metabolism,  pharmacology
Bacillus cereus / chemistry*,  classification,  isolation & purification*,  physiology
Bacterial Proteins / chemistry*,  metabolism,  pharmacology
Camellia sinensis / microbiology
DNA, Bacterial / genetics
DNA, Ribosomal / genetics
Molecular Sequence Data
Phenotype
Phylogeny
Plant Diseases / virology
Plants / microbiology,  virology
RNA, Ribosomal, 16S / genetics
RNA, Viral / metabolism
Sequence Analysis, DNA
Tea / microbiology
Temperature
Tobacco Mosaic Virus / drug effects,  physiology*
Chemical
Reg. No./Substance:
0/Antiviral Agents; 0/Bacterial Proteins; 0/DNA, Bacterial; 0/DNA, Ribosomal; 0/RNA, Ribosomal, 16S; 0/RNA, Viral; 0/Tea

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