Document Detail

Isolation and characterization of Tn5-induced mutants of Pseudomonas paucimobilis UT26 defective in gamma-hexachlorocyclohexane dehydrochlorinase (LinA).
MedLine Citation:
PMID:  7686793     Owner:  NLM     Status:  MEDLINE    
Pseudomonas paucimobilis UT26 grows on gamma-hexachlorocyclohexane (gamma-HCH) as a sole source of carbon and energy. Tn5 mutation was introduced into UT26, and two kinds of mutants defective in gamma-HCH degradation were phenotypically isolated; one (UT64) completely lacked the activity to degrade gamma-HCH, while the other (UT61) retained a very low level of activity. Tagging and sequencing analysis showed that both mutants had a Tn5 insertion at the same site of the linA (gamma-HCH dehydrochlorinase encoding) gene. However, UT61 had an additional rearrangement, which could be the cause of its retaining a low level of activity. An in vitro complementation test with a crude extract from UT64 plus partially purified LinA protein showed that LinA was essential not only for the first-step reaction (gamma-HCH to gamma-pentachloro-cyclohexene; gamma-PCCH), but also for the second-step reaction (gamma-PCCH to compound B) of gamma-HCH degradation in UT26.
Y Nagata; R Imai; A Sakai; M Fukuda; K Yano; M Takagi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  57     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  1993 May 
Date Detail:
Created Date:  1993-08-06     Completed Date:  1993-08-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  703-9     Citation Subset:  B    
Department of Agricultural Chemistry, University of Tokyo, Japan.
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MeSH Terms
Amino Acid Sequence
Base Sequence
Blotting, Southern
Culture Media
DNA Transposable Elements*
Gene Expression Regulation, Bacterial
Genetic Complementation Test
Lindane / metabolism*
Lyases / genetics*,  metabolism
Molecular Sequence Data
Pseudomonas / enzymology,  genetics*
Restriction Mapping
Reg. No./Substance:
0/Culture Media; 0/DNA Transposable Elements; 58-89-9/Lindane; EC 4.-/Lyases; EC

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