Document Detail


Isolation and characterization of CD276+/HLA-E+ human sub-endocardial mesenchymal stem cells from chronic heart failure patients: analysis of differentiative potential and immunomodulatory markers expression.
MedLine Citation:
PMID:  23013234     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Abstract: Mesenchymal stem cells (MSC) are virtually present in all postnatal organs as well as in perinatal tissues. MSC can be differentiated towards severalmature cytotypes and interestingly hold potentially relevant immunomodulatory features. Myocardial infarction results in severe tissue damage, cardiomyocytes loss, and eventually heart failure. Cellular cardiomyoplasty represents a promising approach for myocardial repair. Clinical trials using MSC are underway for a number of heart diseases, even if their outcomes are hampered by low long-term improvements and the possible presence of complications related to cellular therapy administration. Therefore elucidating the presence and role of MSC which reside in the post-infarct human heart should provide essential alternatives for therapy. In the present paper we show a novel method to reproducibly isolate and culture MSC from the sub-endocardial zone of human left ventricle from patients undergoing heart transplant for post-infarct chronic heart failure. By using both immunocytochemistry and RT-PCR, we demonstrated that these cells do express key MSC markers, do express heart-specific transcription factors in their undifferentiated state, while lacking strictly cardiomyocyte-specific proteins. Moreover, these cells do express immunomodulatory molecules which should disclose their further potential in immune modulation processes in the post-infarct microenvironment. Standard MSC trilineage differentiation experiments were also performed. The present paper adds new data on the basic biological features of heart-resident MSC which populate the organ following myocardial infarction. The use of heart-derived MSC to promote in-organ repair or as a cellular source for cardiomyoplasty is a fascinating and challenging task, which deserves further research efforts.
Authors:
Rita Anzalone; Simona Corrao; Melania Lo Iacono; Tiziana Loria; Tiziana Corsello; Francesco Cappello; Antonino Di Stefano; Pantaleo Giannuzzi; Giovanni Zummo; Felicia Farina; Giampiero La Rocca
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-26
Journal Detail:
Title:  Stem cells and development     Volume:  -     ISSN:  1557-8534     ISO Abbreviation:  Stem Cells Dev.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101197107     Medline TA:  Stem Cells Dev     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Università di Palermo, Sezione di Anatomia Umana, Dip. Biomedicina Sperimentale e Neuroscienze Cliniche, Palermo, PA, Italy; ranzalone@hotmail.com.
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