Document Detail


Isolation and characterization of the Candida albicans MOT2 gene.
MedLine Citation:
PMID:  11270411     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A putative Candida albicans homologue of Saccharomyces cerevisiae MOT2 (modulator of transcription) has been cloned and analyzed. A cDNA fragment corresponding to a portion of S. cerevisiae MOT2 was used to isolate a similar C. albicans gene (CaMOT2). CaMOT2 is comprised of two exons of 50 bp and 1,714 bp, respectively, with a single 82 bp intron located near the 5' end of the gene. The gene encodes a protein (CaMot2p) with an estimated mass of 67 kDa. The 5' region of the gene shows sequence homology with S. cerevisiae MOT2, whereas no significant similarity was observed in the 3' region. Similarly, the N-terminal portion of C. albicans Mot2p exhibits approximately 80% homology with S. cerevisiae Mot2p, while no significant homology to any known protein was observed in the carboxy-terminal half of the C. albicans protein. The N-terminal portion of CaMot2p contains a cysteine-rich domain (amino acids 18-62). The distribution of the cysteine residues identifies CaMot2p as a zinc-finger protein. The data suggest two potential Zn-binding sites, similar to the arrangement found in S. cerevisiae. Reverse-transcriptase polymerase chain reaction was used to compare the level of CaMOT2 expression between C. albicans grown in vitro and growth during in vivo infection in the rat model of oral candidiasis. The results showed CaMOT2 is down-regulated during growth in the rat oral cavity compared to in vitro culture. Although the function of C. albicans MOT2 has not been determined, comparison to S. cerevisiae MOT2 suggests the gene product may act as a general negative regulator.
Authors:
X J Zhao; R A Calderone; K E Krueger; G Choi; R L Cihlar
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Medical mycology     Volume:  39     ISSN:  1369-3786     ISO Abbreviation:  Med. Mycol.     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-03-28     Completed Date:  2001-09-06     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  9815835     Medline TA:  Med Mycol     Country:  England    
Other Details:
Languages:  eng     Pagination:  81-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Candida albicans / genetics*
Candidiasis, Oral / microbiology
Cloning, Molecular
Culture Media
DNA, Fungal
Fungal Proteins / genetics*
Gene Expression Regulation, Fungal
Genes, Fungal
Humans
Molecular Sequence Data
Rats
Rats, Sprague-Dawley
Repressor Proteins*
Reverse Transcriptase Polymerase Chain Reaction
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae Proteins*
Sequence Homology, Amino Acid
Grant Support
ID/Acronym/Agency:
P01 AI37251/AI/NIAID NIH HHS; R01 CA88456/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media; 0/DNA, Fungal; 0/Fungal Proteins; 0/MOT2 protein, S cerevisiae; 0/Repressor Proteins; 0/Saccharomyces cerevisiae Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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