| Isolation and characterization of a C12-lipopeptide produced by Bacillus subtilis HSO 121. | |
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MedLine Citation:
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PMID: 18265435 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new lipopeptide with C12 fatty acid has been isolated from the cell broth of Bacillus subtilis HSO121 by chromatographic methods, which is believed to be the homologue of lipopeptides. The fatty acid portion was methylated and analyzed by GC/MS, ESI Q-TOF MS and 1H-NMR. The peptide portion, of which the amino acid composition was obtained by HPLC combined with a phenyl isothiocyanate (PITC) derivatization methods, was analyzed by ESI Q-TOF MS. Comparing the obtained results with surfactin C13 showed that the new lipopeptide has a peptide moiety similar to that of surfactin and the difference exists in the fatty acid portion, which is an iso-C12 beta-hydroxy fatty acid. The critical micelle concentration (CMC) of this new homologue is estimated to be 6.27 x 10(-5) mol/l in 10 mmol/l phosphate buffer solution (PBS, pH 8.0) at 30 degrees C, and the surface tension at CMC (gamma CMC) achieved is as little as 27.71 mN/m. The hemolytic activities of the C12-lipopeptide on 2% human erythrocytes showed a HC50 of 26.5 micromol/l. |
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Authors:
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Xiang-Yang Liu; Shi-Zhong Yang; Bo-Zhong Mu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of peptide science : an official publication of the European Peptide Society Volume: 14 ISSN: 1075-2617 ISO Abbreviation: J. Pept. Sci. Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-06-30 Completed Date: 2008-10-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9506309 Medline TA: J Pept Sci Country: England |
Other Details:
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Languages: eng Pagination: 864-75 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2008 European Peptide Society and John Wiley & Sons, Ltd. |
Affiliation:
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Laboratory for Advanced Materials and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacillus subtilis
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chemistry*,
metabolism* Carbon / chemistry* Erythrocytes / drug effects Gas Chromatography-Mass Spectrometry Hemolysis / drug effects Humans Lipoproteins / biosynthesis, chemistry*, isolation & purification*, pharmacology Magnetic Resonance Spectroscopy Mass Spectrometry Molecular Structure Surface Properties |
| Chemical | |
Reg. No./Substance:
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0/Lipoproteins; 7440-44-0/Carbon |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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