Document Detail


Isolation and characterization of 8 microsatellite loci from Chrysophyllum gonocarpum (Sapotaceae).
MedLine Citation:
PMID:  23420366     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Chrysophyllum gonocarpum is a tropical tree species that is very important in the recovery of heterogeneous forests and of degraded areas of permanent preservation. We identified microsatellite loci for C. gonocarpum to assess the genetic variability and the patterns of the population structure of the species. We isolated 8 microsatellite primers by using CT- and GT-enriched genomic libraries. We detected 2-4 alleles with 2.9 alleles per locus on average, by polymerase chain reaction. Test for cross-amplification showed that some loci were successfully amplified in 2 other Chrysophyllum species. The microsatellites can be used to assess the genetic diversity and population structure of C. gonocarpum. Some primer pairs can be amplified in C. marginatum and C. splendens.
Authors:
L A Rodrigues; E A Ruas; N C Signori; B F Costa; D C L Farias-Missão; B G Vieira; A R O Conson; C L Chaves; J Delfini; C F Ruas; P M Ruas
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Publication Detail:
Type:  Journal Article     Date:  2013-02-08
Journal Detail:
Title:  Genetics and molecular research : GMR     Volume:  12     ISSN:  1676-5680     ISO Abbreviation:  Genet. Mol. Res.     Publication Date:  2013  
Date Detail:
Created Date:  2013-02-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101169387     Medline TA:  Genet Mol Res     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  416-9     Citation Subset:  IM    
Affiliation:
Departamento de Agronomia, Centro de Ciências Agrárias, Universidade Estadual de Londrina, Londrina, PR, Brasil.
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