Document Detail


Isolation and characterization of 23 carbofuran-degrading bacteria from soils from distant geographical areas.
MedLine Citation:
PMID:  11069636     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this work was to isolate, identify and type carbofuran-degrading bacteria from two geographically distant soils. Restriction Fragment Length Polymorphism (RFLP) patterns of the 16S rRNA gene and partial 16S rRNA sequence analysis were used to classify the 23 isolates obtained. Nine of them showed high similarity to Pseudomonas strains, seven showed similarity to the Flexibacter/Cytophaga/Bacteroides group and the remainder showed similarity to other bacterial genera. Isolates within the same group were sub-typed by comparing partial 16S rRNA sequences and SDS-PAGE analysis of their total protein profiles. Many of the UK isolates showed similarity to the Pseudomonas genera, while most of the Greek isolates showed similarity to the Flexibacter/Cytophaga/Bacteroides group. Only two Chrysobacterium strains isolated from both the UK and Greek soils were identical.
Authors:
D G Karpouzas; J A Morgan; A Walker
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Letters in applied microbiology     Volume:  31     ISSN:  0266-8254     ISO Abbreviation:  Lett. Appl. Microbiol.     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2000-12-27     Completed Date:  2000-12-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  353-8     Citation Subset:  IM    
Affiliation:
Department of Soil and Environment Sciences, Horticulture Research International, Wellesbourne, Warwick, UK.
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MeSH Terms
Descriptor/Qualifier:
Bacteria / classification,  isolation & purification,  metabolism*
Bacteroides / isolation & purification
Carbofuran / metabolism*
Geography
Great Britain
Greece
Molecular Sequence Data
Polymorphism, Restriction Fragment Length
Pseudomonas / isolation & purification
RNA, Ribosomal, 16S / genetics
Soil Microbiology*
Chemical
Reg. No./Substance:
0/RNA, Ribosomal, 16S; 1563-66-2/Carbofuran

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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