Document Detail


Isolation and characterisation of radioresistant human KB carcinoma cells in vitro.
MedLine Citation:
PMID:  9659517     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to investigate the radioresistance mechanism in human carcinoma cells, we isolated the radioresistant cells from a human KB carcinoma cell line by various methods. Although the radioresistant cells were not isolated by the repeated X-irradiation method, pretreatment of the cells with mutagens including N-methyl-N'nitro-N-nitrosoguanidine and 4-nitroquinoline 1-oxide induced radioresistance of 1.50 to 3.75-fold, as judged by the D0 ratio to parental KB cells. When three representative radioresistant cell clones with stable growth isolated by a different method were examined for cross-resistance to anticancer drugs, each cell clone exhibited resistance to one, five and two anticancer drugs, including mitomycin C, bleomycin and methotrexate, respectively. Among them, the cell line N10, showing stable plating efficiency and population doubling time, was further characterised. Consequently, immunocytochemistry, Western blotting and flow cytometry revealed that N10 expressed a higher level of mutant p53 protein than did parental KB, suggesting the involvement of mutant p53 protein in radioresistance of N10.
Authors:
T Yanagisawa; M Urade; Y Takahashi; H Kishimoto; K Sakurai
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Oral oncology     Volume:  34     ISSN:  1368-8375     ISO Abbreviation:  Oral Oncol.     Publication Date:  1998 Jan 
Date Detail:
Created Date:  1998-07-23     Completed Date:  1998-07-23     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  9709118     Medline TA:  Oral Oncol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  30-8     Citation Subset:  IM    
Affiliation:
Department of Dentistry and Oral Surgery, Hyogo College of Medicine, Japan.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents / pharmacology
Blotting, Western
Carcinoma, Squamous Cell / pathology*
Cell Survival / drug effects,  radiation effects
Flow Cytometry
Humans
Immunoenzyme Techniques
Mouth Neoplasms / pathology*
Neoplasm Proteins / metabolism
Radiation Tolerance*
Tumor Cells, Cultured / radiation effects
Tumor Suppressor Protein p53 / metabolism
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Neoplasm Proteins; 0/Tumor Suppressor Protein p53

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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