Document Detail

Isolation and characterisation of the lipopolysaccharide from Xanthomonas hortorum pv. vitians.
MedLine Citation:
PMID:  10564788     Owner:  NLM     Status:  MEDLINE    
Xanthomonas hortorum pv. vitians is a Gram-negative bacterium that acts as the causative agent of bacterial leaf spot and headrot in lettuce. The lipopolysaccharide (LPS) of this bacterium is suspected to be an important molecule for adhesion to the plants. We have isolated the LPS, prepared the lipid A and the polysaccharide moieties thereof, and characterised all preparations by compositional analysis. Main sugar components are rhamnose and 3-acetamido-3,6-dideoxy-galactose which presumably furnish the O-specific polysaccharide. Other sugars are mannose, glucose, 6-deoxygalactose (fucose), and galacturonic acid, which should be core region constituents, and glucosamine, which builds up the carbohydrate backbone of lipid A. The LPS contains several phosphate groups, most of which are present in the core region. The main fatty acids in the lipid A are C10:0, 3-OH-C10:0 and 3-OH-C12:0. The latter is the only amide-linked fatty acid. Two fatty acids present in small amounts were identified, C8:0 and C11:0.
A Molinaro; R Lanzetta; A Evidente; M Parrilli; O Holst
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  181     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  1999 Dec 
Date Detail:
Created Date:  2000-02-03     Completed Date:  2000-02-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  49-53     Citation Subset:  IM    
Dipartimento di Chimica Organica e Biologica, Università di Napoli Federico II, I-80134, Naples, Italy.
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MeSH Terms
Lettuce / microbiology*
Lipid A / analysis
Lipopolysaccharides / chemistry*,  isolation & purification*
Plant Diseases / microbiology
Polysaccharides / analysis
Xanthomonas / chemistry*,  pathogenicity
Reg. No./Substance:
0/Lipid A; 0/Lipopolysaccharides; 0/Polysaccharides

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