Document Detail

Isolation and characterisation of cell wall polysaccharides from cocoa (Theobroma cacao L.) beans.
MedLine Citation:
PMID:  10805455     Owner:  NLM     Status:  MEDLINE    
Cell wall material (CWM) was prepared from sun-dried cocoa (Theobroma cacao L.) bean cotyledons before and after fermentation. The monosaccharide composition of the CWM was identical for unfermented and fermented beans. Polysaccharides of the CWM were solubilised by sequential extraction with 0.05 M trans-1,2-diaminocyclohexane-N,N,N',N'-tetraacetic acid (CDTA), 0.05 M Na2CO3, and 1 M, 4 M and 8 M KOH. The non-cellulosic sugar composition for each fraction was similar for unfermented and fermented samples, indicating that fermentation caused no significant modification of the structural features of individual cell wall polysaccharides. Pectic polysaccharides accounted for 60% of the cell wall polysaccharides but only small amounts could be solubilised in solutions of CDTA, Na2CO3, and 1 M and 4 M KOH. The bulk of the pectic polysaccharides were solubilised in 8 M KOH and were characterised by a rhamnogalacturonan backbone heavily substituted with side-chains of 5-linked arabinose and 4-linked galactose. Linkage analysis indicated the presence of additional acidic polysaccharides, including a xylogalacturonan and a glucuronoxylan. Cellulose, xyloglucan and a galactoglucomannan accounted for 28%, 8% and 3% of the cell wall polysaccharides, respectively. It is concluded that the types and structural features of cell wall polysaccharides in cocoa beans resemble those found in the parenchymatous tissue of many fruits and vegetables rather than those reported for many seed storage polysaccharides.
R J Redgwell; C E Hansen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Planta     Volume:  210     ISSN:  0032-0935     ISO Abbreviation:  Planta     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-07-28     Completed Date:  2000-07-28     Revised Date:  2002-11-01    
Medline Journal Info:
Nlm Unique ID:  1250576     Medline TA:  Planta     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  823-30     Citation Subset:  IM; S    
Nestlé Research Center, Nestec Ltd., Lausanne, Switzerland.
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MeSH Terms
Cacao / chemistry*
Cell Wall / chemistry*
Chemical Fractionation
Chromatography, Gel
Monosaccharides / analysis
Pectins / analysis
Polysaccharides / analysis*,  isolation & purification
Potassium Compounds
Reg. No./Substance:
0/Hydroxides; 0/Monosaccharides; 0/Pectins; 0/Polysaccharides; 0/Potassium Compounds; 1310-58-3/potassium hydroxide; 8024-50-8/hemicellulose; 9000-69-5/pectin

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