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Isolation of centrosomes from cultured Mammalian cells.
MedLine Citation:
PMID:  21356896     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
INTRODUCTIONThe centrosome is a cell's primary microtubule-organizing center. In most mammalian cells, the centrosome is composed of a pair of centrioles and surrounding pericentriolar material. The centrosome is duplicated exactly once per cell cycle such that at the onset of mitosis, a cell has two centrosomes, which serve as poles of the mitotic spindle. During cytokinesis, one centrosome is segregated to each daughter cell. This protocol describes the isolation of centrosomes from asynchronous cells, and thus the purified material will consist primarily of interphase centrosomes. Isolated centrosomes can be used in a variety of assays, including studies of microtubule function and the identification of centrosome-associated proteins and their interactions.
Authors:
Thomas E Meigs; Daniel D Kaplan
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Publication Detail:
Type:  Journal Article     Date:  2008-08-01
Journal Detail:
Title:  CSH protocols     Volume:  2008     ISSN:  -     ISO Abbreviation:  CSH Protoc     Publication Date:  2008  
Date Detail:
Created Date:  2011-03-01     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101280522     Medline TA:  CSH Protoc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  pdb.prot5039     Citation Subset:  -    
Affiliation:
Department of Biology, University of North Carolina at Asheville, Asheville, NC 28804, USA.
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