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Isolation of cells from the feto-maternal interface.
MedLine Citation:
PMID:  22470137     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The mucosal lining of the human uterus is host to a specialized population of leukocytes, which, during pregnancy, interact with invading placental cells (trophoblast) of fetal origin. Of particular interest are uterine natural killer cells, which account for around 70% of the leukocytes at this site during the first trimester of pregnancy, and seem to be specially adapted to recognize invading trophoblast cells. In order to understand the interactions between mucosal immune cells and trophoblast, and those among the immune cells themselves, it is useful to be able to isolate and culture these cells. Here, we describe protocols for the isolation of leukocytes, stromal cells, and trophoblast cells from the feto-maternal interface. Curr. Protoc. Immunol. 97:7.40.1-7.40.11. © 2012 by John Wiley & Sons, Inc.
Authors:
Victoria Male; Lucy Gardner; Ashley Moffett
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current protocols in immunology / edited by John E. Coligan ... [et al.]     Volume:  Chapter 7     ISSN:  1934-368X     ISO Abbreviation:  Curr Protoc Immunol     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-04-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9101651     Medline TA:  Curr Protoc Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit7.40     Citation Subset:  IM    
Affiliation:
Department of Pathology, University of Cambridge, Cambridge, United Kingdom.
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