Document Detail


Isolation of cell lines that show novel, murine leukemia virus-specific blocks to early steps of retroviral replication.
MedLine Citation:
PMID:  16188999     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to identify cellular proteins required for early stages of retroviral replication, a high volume screening with mammalian somatic cells was performed. Ten pools of chemically mutagenized Chinese hamster ovary (CHO-K1) cells were challenged with a murine leukemia virus (MLV) vector pseudotyped with the vesicular stomatitis virus glycoprotein (VSV-G), and cells that failed to be transduced were enriched by cell sorting. Each pool yielded a clonally derived cell line with a 5-fold or greater resistance to virus infection, and five cell lines exhibited a >50-fold resistance. These five cell lines were efficiently infected by a human immunodeficiency virus vector pseudotyped with VSV-G. When engineered to express the TVA receptor for subgroup A avian sarcoma and leukosis virus (ASLV-A), the five cell lines were resistant to infection with a MLV vector pseudotyped with the ASLV-A envelope protein but were fully susceptible to infection with an ASLV-A vector. Thus, the defect in these cells resides after virus-cell membrane fusion and, unlike those in other mutant cell lines that have been described, is specific for the MLV core. To identify the specific stages of MLV infection that are impaired in the resistant cell lines, real-time quantitative PCR analyses were employed and two phenotypic groups were identified. Viral infection of three cell lines was restricted before reverse transcription; in the other two cell lines, it was blocked after reverse transcription, nuclear localization, and two-long terminal repeat circle formation but before integration. These data provide genetic evidence that at least two distinct intracellular gene products are required specifically for MLV infection. These cell lines are important tools for the biochemical and genetic analysis of early stages in retrovirus infection.
Authors:
James W Bruce; Kenneth A Bradley; Paul Ahlquist; John A T Young
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of virology     Volume:  79     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-09-28     Completed Date:  2005-11-08     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  12969-78     Citation Subset:  IM    
Affiliation:
Institute for Molecular Virology, University of Wisconsin, Madison, 53706-1596, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
CHO Cells
Clone Cells
Cricetinae
Gene Expression Regulation, Viral*
Leukemia Virus, Murine / genetics,  physiology*
Reverse Transcription
Virus Integration
Virus Replication
Grant Support
ID/Acronym/Agency:
CA22443/CA/NCI NIH HHS; CA70810/CA/NCI NIH HHS; T32 CA009075/CA/NCI NIH HHS
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