Document Detail


Isolation and cell culture of the epithelial cells of cauda epididymidis of the bull.
MedLine Citation:
PMID:  4063439     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A simple and rapid method has been described to isolate the epithelial cells of cauda epididymidis of adult bull. Perfusion of the lumen of the cauda epididymidis with 1 mg/ml collagenase in calcium- and magnesium-free Hank's balanced salt solution and incubation of the tissue at 37 degrees C for 90 min releases the principal and basal cells into the lumen. Several individual epithelial cells and cell aggregates without the contamination of stromal or smooth muscle cells can be flushed out at the end of incubation. The isolated epithelial cells, suspended in Dulbecco's medium with 10% horse serum, attach to plastic dishes within 3 h after seeding the cells and proliferate to form a monolayer in approximately 8-12 days. The electron microscopic study and immunostaining of the cultured epithelial cells indicate that the cultured cells are principal cells. The basal cells of the intact cauda epididymidis of bull show within their cytoplasm the presence of varying amounts of "lipid-like" material often closely associated with whorls of membrane.
Authors:
M S Joshi
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biology of reproduction     Volume:  33     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  1985 Aug 
Date Detail:
Created Date:  1986-01-08     Completed Date:  1986-01-08     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  187-200     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle / anatomy & histology
Cells, Cultured
Epididymis / cytology*,  ultrastructure
Epithelium
Male
Grant Support
ID/Acronym/Agency:
BRSG S 07 RR 05407-02/RR/NCRR NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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