Document Detail


Isolation of a cDNA clone encoding the amino-terminal region of human apolipoprotein B.
MedLine Citation:
PMID:  3513177     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A partial cDNA clone for the B-26 region of apolipoprotein B was isolated from an adult human liver DNA library by screening with an oligonucleotide probe derived from amino-terminal protein sequence obtained from purified B-26 peptide. Antisera against a synthetic 17-residue peptide whose amino acid sequence was encoded by the clone cross-reacts with apolipoproteins B-26, B-100, and B-48, but not with B-74. The nucleotide sequence immediately upstream from the amino terminus of B-26 codes for an apparent signal sequence, implying that the B-26 moiety is in an amino-terminal locus in the B-100 protein. That this sequence represents a 5' end region is further supported by primer extension analysis using a fragment of the cDNA clone and by S1 nuclease protection experiments using the corresponding region in a genomic clone.
Authors:
A A Protter; D A Hardman; J W Schilling; J Miller; V Appleby; G C Chen; S W Kirsher; G McEnroe; J P Kane
Related Documents :
24414097 - Mating type gene (mat1-2) of trichophyton verrucosum.
8206387 - A dog growth hormone cdna codes for a mature protein identical to pig growth hormone.
25004147 - A comparison of molecular markers to detect lutzomyia longipalpis naturally infected wi...
23079977 - Development and characterization of dna microsatellite primers for buriti (mauritia fle...
1791857 - Nucleotide sequence of p49a, a genomic y-specific probe with potential utilization in s...
2837737 - The genome of plasmodium cynomolgi is partitioned into separable domains which appear t...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  83     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1986 Mar 
Date Detail:
Created Date:  1986-04-10     Completed Date:  1986-04-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1467-71     Citation Subset:  IM    
Data Bank Information
Bank Name/Acc. No.:
GENBANK/M12681
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Apolipoproteins B / genetics*,  immunology
Base Sequence
Chromosome Mapping
Cloning, Molecular
DNA / genetics
Genes
Humans
Immunosorbent Techniques
RNA, Messenger / genetics
Grant Support
ID/Acronym/Agency:
HL 14237/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Apolipoproteins B; 0/RNA, Messenger; 9007-49-2/DNA
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Induction of c-myc expression in human B lymphocytes by B-cell growth factor and anti-immunoglobulin...
Next Document:  Altered expression of G1-specific genes in human malignant myeloid cells.