Document Detail


Isolation of bronchial mucins from cystic fibrosis sputum by use of citraconic anhydride.
MedLine Citation:
PMID:  7116358     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Citraconylation was used to solubilize cystic fibrosis sputum and to dissociate its mucus glycoproteins from extraneous proteins. The mucin fraction was isolated by precipitation with Cetavlon, and characterized in terms of amino acid and carbohydrate composition. The data suggest that, in determining the physical properties of glycoproteins of cystic fibrosis mucus, aggregation by noncovalent forces may be as important as (or more important than) disulfide bonds.
Authors:
C E Snyder; C E Nadziejko; A Herp
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Carbohydrate research     Volume:  105     ISSN:  0008-6215     ISO Abbreviation:  Carbohydr. Res.     Publication Date:  1982 Jul 
Date Detail:
Created Date:  1982-12-02     Completed Date:  1982-12-02     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0043535     Medline TA:  Carbohydr Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  87-93     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Bronchi / secretion*
Carbohydrates / analysis
Citraconic Anhydrides
Cystic Fibrosis / metabolism*
Glycoproteins / isolation & purification
Humans
Mucins / isolation & purification*,  secretion
Sputum / analysis*
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Carbohydrates; 0/Citraconic Anhydrides; 0/Glycoproteins; 0/Mucins; 616-02-4/citraconic anhydride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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