| Isolation and biochemical characterization of frog retinal pigment epithelium cells. | |
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MedLine Citation:
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PMID: 3721796 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The structure and function of the photoreceptor cell depends on the renewal of its outer segment. Phagocytosis of the rod outer segments by RPE is an essential part of the renewal process. Several methods have been reported in order to isolate RPE cells; however, the isolated cells are heavily contaminated by other cell types, mainly erythrocytes and rod outer segments. The primary aim of this study was the isolation of pure and viable frog RPE cells. Cells were dissociated in a calcium-free Krebs bicarbonate medium and purified by centrifugation in a ficoll density gradient. Viability of the purified cells assessed by trypan blue dye exclusion was 95%. The metabolic activity of the cells was tested by several parameters: RPE cells consume oxygen at a rate of 11.5 ngatoms/min/mg protein, transport and metabolize 14C-glucose by a sodium dependent mechanism, and are able as well to accumulate 14C-leucine and incorporate it into proteins. Results obtained in this study indicate that our isolation procedure yields a more intact preparation of RPE than those described previously; hence, it may be helpful in elucidating the biochemical and metabolic parameters involved in pigment epithelium physiology. |
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Authors:
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R Salceda |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Investigative ophthalmology & visual science Volume: 27 ISSN: 0146-0404 ISO Abbreviation: Invest. Ophthalmol. Vis. Sci. Publication Date: 1986 Jul |
Date Detail:
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Created Date: 1986-08-08 Completed Date: 1986-08-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1172-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Survival Glucose / metabolism Pigment Epithelium of Eye / metabolism, ultrastructure* |
| Chemical | |
Reg. No./Substance:
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50-99-7/Glucose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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