| Isolation and biochemical characterization of extracellular polymeric secretions (EPS) from modern soft marine stromatolites (Bahamas) and its inhibitory effect on CaCO3 precipitation. | |
| | |
MedLine Citation:
|
PMID: 11934077 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Bahamian soft marine stromatolites consist of cyanobacterial biofilms and carbonate sand grains (ooids) embedded in their extracellular polymeric secretions (EPS). EPS were isolated from natural marine stromatolites and the laboratory cultured stromatolite forming cyanobacterium isolate Schizothix sp. Laboratory investigations were conducted to examine biochemical characteristics and the role of EPS in the inhibition of CaCO3 precipitation. EPS consisted of acid polysaccharides and proteins. SDS-PAGE and amino acid analysis suggested that EPS from both soft marine stromatolite and Schizothrix sp. mat contained small proteins (38 kD and 45 kD) enriched in aspartic acid and glutamic acid. Also, immuno blotting suggested that natural EPS contain high molecular weight acid polysaccharide (500 k) which may represent cross-linked products of laboratory cultured Schizothrix sp. acid polysaccharide (300 k). EPS from both soft marine stromatolite and laboratory cultured Schizothrix sp. inhibited CaCO3 precipitation in vitro, as determined using pH drift assays examining pH decrease which occur in response to CaCO3 precipitation. PH drift assays of enzymatically and chemically modified EPS isolated from soft marine stromatolite and laboratory cultured Schizothrix sp. indicated that both uronic acids and protein fractions may be involved in the inhibition of CaCO3 precipitation. |
| | |
Authors:
|
Tomohiro Kawaguchi; Alan W Decho |
Related Documents
:
|
14995147 - Anatomy and cell wall polysaccharides of almond (prunus dulcis d. a. webb) seeds. 12473097 - A polymer with a backbone of 3-deoxy-d-glycero-d-galacto-non-2-ulopyranosonic acid, a t... 17931857 - Dietary fibre components and pectin chemical features of peels during ripening in banan... 6293397 - Murein and lipopolysaccharide biosynthesis in synchronized cells of escherichia coli k ... 2105377 - An improved hplc-electrochemical detection method for measuring brain levels of 5-s-cys... 14675637 - Soluble microbial products formation in anaerobic chemostats in the presence of toxic c... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
|
Title: Preparative biochemistry & biotechnology Volume: 32 ISSN: 1082-6068 ISO Abbreviation: Prep. Biochem. Biotechnol. Publication Date: 2002 Feb |
Date Detail:
|
Created Date: 2002-04-05 Completed Date: 2002-10-02 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 9607037 Medline TA: Prep Biochem Biotechnol Country: United States |
Other Details:
|
Languages: eng Pagination: 51-63 Citation Subset: IM |
Affiliation:
|
Department of Environmental Health Sciences, School of Public Health, University of South Carolina, Columbia 29208, USA. tkawaguchi@sophe.sph.sc.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acids
/
analysis Antibodies / immunology Bacterial Proteins / analysis, chemistry Bahamas Biofilms Biopolymers / chemistry, immunology, isolation & purification*, secretion* Calcium Carbonate / chemistry* Chemical Precipitation Cyanobacteria / chemistry, immunology, metabolism* Electrophoresis, Polyacrylamide Gel Hydrogen-Ion Concentration Immunoblotting Polysaccharides / analysis, chemistry Silicon Dioxide Time Factors Water Microbiology |
| Chemical | |
Reg. No./Substance:
|
0/Amino Acids; 0/Antibodies; 0/Bacterial Proteins; 0/Biopolymers; 0/Polysaccharides; 471-34-1/Calcium Carbonate; 7631-86-9/Silicon Dioxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Purification of turnip peroxidase and its kinetic properties.
Next Document: HPLC preparation of fish waste hydrolysate fractions. Effect on guinea pig ileum and ACE activity.