Document Detail

Isolation of bacilysin and a new amino acid from culture filtrates of Bacillus subtilis.
MedLine Citation:
PMID:  4991475     Owner:  NLM     Status:  MEDLINE    
1. Bacilysin, a labile dipeptide antibiotic that lyses growing staphylococci, was isolated from culture fluids of Bacillus subtilis by a process giving higher yields than those previously obtained. 2. The process involves adsorption on a cation-exchange resin and elution with aqueous trimethylamine, separation from neutral amino acids and glutamic acid by chromatography on DEAE-Sephadex at pH8.7 and separation from other neutral peptides by chromatography in aqueous propan-2-ol on Sephadex G-25. 3. A new amino acid, which is chemically related to bacilysin, was isolated from the fraction containing neutral amino acids. 4. Two substances that yield alanine on hydrolysis, in addition to bacilysin, were obtained from the neutral peptide fraction.
J E Walker; E P Abraham
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  118     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1970 Jul 
Date Detail:
Created Date:  1971-01-13     Completed Date:  1971-01-13     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  557-61     Citation Subset:  IM    
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MeSH Terms
Alanine / analysis
Amino Acids / isolation & purification*
Anti-Bacterial Agents / isolation & purification*
Bacillus subtilis*
Chromatography, Ion Exchange
Culture Media / analysis*
Dipeptides / isolation & purification
Peptides / analysis
Reg. No./Substance:
0/Amines; 0/Amino Acids; 0/Anti-Bacterial Agents; 0/Culture Media; 0/Dipeptides; 0/Glutamates; 0/Peptides; 56-41-7/Alanine; 9004-54-0/Dextrans

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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