Document Detail

Isolation and anti-HIV-1 activity of a new sesquiterpene lactone from Calocephalus brownii F. Muell.
MedLine Citation:
PMID:  23297755     Owner:  NLM     Status:  Publisher    
As part of our ongoing collaborative effort to discover potential anti-HIV-1 agents from plants, the CH(2)Cl(2) extract of Calocephalus brownii F. Muell. was phytochemically investigated, which resulted in the isolation of two sesquiterpene lactones of the cis-fused guaianolides type, one new identified as 1α-hydroxy-3βH-3α,4α-epoxy-4,10-dimethyl-5αH,7αH,8βH,10αH-guai-11(13)-ene-7,8-olide (1) and one known identified 1αH-4α-hydroxy-4,10-dimethyl-5αH,7αH,8βH,10αH-guai-11(13)-ene-7,8-olide (2). Their structures were elucidated on the bases of IR, UV, 1D-NMR, 2D-NMR, DIFNOE and high-resolution ESI-TOF-MS. The anti-HIV-1 activity was evaluated and revealed that STLs (1 and 2) caused a reduction for the viability of mock-infected MT-4 cells by CC(50) = 29.1 and 0.5 μg/mL, respectively, and caused 50% protection of MT-4 cells against HIV-1 induced cytopathogeneticy by EC(50) = 29.1 and 0.5 μg/mL, respectively, as compared with Efavirenz (EFV) as positive control that showed a CC(50) = 11.6 and EC(50) = 0.0006 μg/mL, using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide method.
Magdy M D Mohammed; Lars P Christensen; Paolo L Colla
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-9
Journal Detail:
Title:  Natural product research     Volume:  -     ISSN:  1478-6427     ISO Abbreviation:  Nat. Prod. Res.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101167924     Medline TA:  Nat Prod Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
a Danish Institute of Agricultural Sciences (DIAS), Department of Food Science, Research Center Aarslev , Aarhus University , Kirstinebjergvej 10, DK-5792 , Aarslev , Denmark.
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