| Isolation and annotation of 10828 putative full length cDNAs from indica rice. | |
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MedLine Citation:
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PMID: 16315595 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We reported the isolation and identification of 10828 putative full-length cDNAs (FL-cDNA) from an indica rice cultivar, Minghui 63, with the long-term goal to isolate all full-length cDNAs from indica genome. Comparison with the databases showed that 780 of them are new rice cDNAs with no match in japonica cDNA database. Totally, 9078 of the FL-cDNAs contained predicted ORFs matching with japonica FL-cDNAs and 6543 could find homologous proteins with complete ORFs. 53% of the matched FL-cDNAs isolated in this study had longer 5'UTR than japonica FL-cDNAs. In silico mapping showed that 9776 (90.28%) of the FL-cDNAs had matched genomic sequences in the japonica genome and 10046 (92.78%) had matched genomic sequences in the indica genome. The average nucleotide sequence identity between the two subspecies is 99.2%. A majority of FL-cDNAs (90%) could be classified with GO (gene ontology) terms based on homology proteins. More than 60% of the new cDNAs isolated in this study had no homology to the known proteins. This set of FL-cDNAs should be useful for functional genomics and proteomics studies. |
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Authors:
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Kabin Xie; Jianwei Zhang; Yong Xiang; Qi Feng; Bin Han; Zhaohui Chu; Shiping Wang; Qifa Zhang; Lizhong Xiong |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Science in China. Series C, Life sciences / Chinese Academy of Sciences Volume: 48 ISSN: 1006-9305 ISO Abbreviation: Sci. China, C, Life Sci. Publication Date: 2005 Oct |
Date Detail:
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Created Date: 2005-11-30 Completed Date: 2008-08-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9611809 Medline TA: Sci China C Life Sci Country: China |
Other Details:
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Languages: eng Pagination: 445-51 Citation Subset: IM |
Affiliation:
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National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research, Wuhan, Huazhong Agricultural University, Wuhan 430070, China |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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DNA, Complementary
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isolation & purification* Gene Library Oryza sativa / genetics* Sequence Analysis, DNA Species Specificity |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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