Document Detail


Isolation of an anaerobic intestinal bacterium capable of cleaving the C-ring of the isoflavonoid daidzein.
MedLine Citation:
PMID:  12070764     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Colonic bacteria were screened for bacteria involved in the conversion of phytoestrogens. A gram-positive anaerobic bacterium, strain HGH 136, capable of conversion of the isoflavonoid daidzein, was isolated and identified as a Clostridium sp. The bacterium cleaved the C-ring of daidzein to produce O-demethylangolensin ( O-Dma). This compound was identified by comparison of the HPLC retention time and UV spectrum of the metabolite with chemically synthesized O-Dma. The identity of the metabolite was confirmed by liquid chromatography-mass spectrometry and NMR using synthetic O-Dma as a standard. The bacterium incubated with synthetic dihydrodaidzein also produced O-Dma. After 3 days of incubation, 28% of added daidzein and 12% of added dihydrodaidzein were converted to O-Dma. This is the first study in which an anaerobic bacterium involved in the ring cleavage of daidzein to produce O-Dma has been identified.
Authors:
Hor-Gil Hur; Richard D Beger; Thomas M Heinze; Jackson O Lay; James P Freeman; Joel Dore; Fatemeh Rafii
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2002-04-16
Journal Detail:
Title:  Archives of microbiology     Volume:  178     ISSN:  0302-8933     ISO Abbreviation:  Arch. Microbiol.     Publication Date:  2002 Jul 
Date Detail:
Created Date:  2002-06-18     Completed Date:  2002-09-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0410427     Medline TA:  Arch Microbiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  8-12     Citation Subset:  IM    
Affiliation:
Department of Environmental Science and Engineering, Kwangju Institute of Science and Technology, Korea.
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MeSH Terms
Descriptor/Qualifier:
Bacteria, Anaerobic / isolation & purification*,  metabolism
Carbon / chemistry,  metabolism*
Chromatography, High Pressure Liquid
Humans
Intestines / microbiology*
Isoflavones / chemistry,  metabolism*
Phylogeny
RNA, Ribosomal, 16S / analysis
Chemical
Reg. No./Substance:
0/Isoflavones; 0/RNA, Ribosomal, 16S; 486-66-8/daidzein; 7440-44-0/Carbon
Comments/Corrections
Erratum In:
Arch Microbiol 2002 Jul;178(1):75

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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