Document Detail

Isolation of acidic acrosin isoinhibitors (BUSI I A, BUSI IB1 and BUSI I B2) from bull seminal plasma.
MedLine Citation:
PMID:  393605     Owner:  NLM     Status:  MEDLINE    
Three natural proteinase isoinhibitors with low isoelectric points BUSI I A (pI = 3.9), BUSI I B1 (pI = 3.4 and BUSI I B2 (pI = 3.7) were isolated from bull seminal plasma by gel filtration on Sephadex G-50 and ion exchange chromatography on DEAE-Sephadex and SE-Sephadex. Isoinhibitors Bl and B2 have identical amino acid composition. Isoinhibitor A contains six amino acid residues less than isoinhibitors B1 and B2. Since sugars have been detected in the isoinhibitors, heterogeneity may also be due to the sugar component. The isoinhibitors show the same inhibitory properties; all of them inhibit acrosin, trypsin and chymotrypsin. Glandular kallikrein is also inhibited, but to a very low extent only. The molecular weight (Mr approximately 8 900) was determined by gel filtration.
D Cechová; V Jonáková; M Havranová; E Sedláková; O Mach
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Hoppe-Seyler's Zeitschrift für physiologische Chemie     Volume:  360     ISSN:  0018-4888     ISO Abbreviation:  Hoppe-Seyler's Z. Physiol. Chem.     Publication Date:  1979 Dec 
Date Detail:
Created Date:  1980-04-17     Completed Date:  1980-04-17     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  2985060R     Medline TA:  Hoppe Seylers Z Physiol Chem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  1759-66     Citation Subset:  IM    
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MeSH Terms
Acrosin / antagonists & inhibitors*
Amino Acids / analysis
Glycoproteins / isolation & purification*,  pharmacology
Molecular Weight
Protease Inhibitors* / isolation & purification*
Semen / analysis*
Species Specificity
Trypsin Inhibitors
Reg. No./Substance:
0/Amino Acids; 0/Glycoproteins; 0/Protease Inhibitors; 0/Trypsin Inhibitors; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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