| Isolation of acidic acrosin isoinhibitors (BUSI I A, BUSI IB1 and BUSI I B2) from bull seminal plasma. | |
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MedLine Citation:
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PMID: 393605 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Three natural proteinase isoinhibitors with low isoelectric points BUSI I A (pI = 3.9), BUSI I B1 (pI = 3.4 and BUSI I B2 (pI = 3.7) were isolated from bull seminal plasma by gel filtration on Sephadex G-50 and ion exchange chromatography on DEAE-Sephadex and SE-Sephadex. Isoinhibitors Bl and B2 have identical amino acid composition. Isoinhibitor A contains six amino acid residues less than isoinhibitors B1 and B2. Since sugars have been detected in the isoinhibitors, heterogeneity may also be due to the sugar component. The isoinhibitors show the same inhibitory properties; all of them inhibit acrosin, trypsin and chymotrypsin. Glandular kallikrein is also inhibited, but to a very low extent only. The molecular weight (Mr approximately 8 900) was determined by gel filtration. |
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Authors:
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D Cechová; V Jonáková; M Havranová; E Sedláková; O Mach |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Hoppe-Seyler's Zeitschrift für physiologische Chemie Volume: 360 ISSN: 0018-4888 ISO Abbreviation: Hoppe-Seyler's Z. Physiol. Chem. Publication Date: 1979 Dec |
Date Detail:
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Created Date: 1980-04-17 Completed Date: 1980-04-17 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985060R Medline TA: Hoppe Seylers Z Physiol Chem Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 1759-66 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acrosin
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antagonists & inhibitors* Amino Acids / analysis Animals Cattle Glycoproteins / isolation & purification*, pharmacology Kinetics Male Molecular Weight Protease Inhibitors* / isolation & purification* Semen / analysis* Species Specificity Swine Trypsin Inhibitors |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Glycoproteins; 0/Protease Inhibitors; 0/Trypsin Inhibitors; EC 3.4.21.10/Acrosin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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