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Isolation of uridine 5'-pyrophosphate glucuronic Acid pyrophosphorylase and its assay using p-pyrophosphate.
MedLine Citation:
PMID:  16659998     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
A procedure was devised to detect and assay uridine 5'-pyrophosphate (UDP)-glucuronic acid pyrophosphorylase in plant extracts. Substrates are UDP-glucuronic acid and (32)P-pyrophosphate, and the (32)P-uridine 5'-triphosphate produced is selectively adsorbed to charcoal. The charcoal adsorption procedure is a modification of that used to determine (32)P-adenosine 5'-triphosphate produced by adenosine 5'-pyrophosphate glucose pyrophosphorylase, and the modification greatly improves the retention of uridine 5'-triphosphate.
Authors:
D B Dickinson; D Hyman; J W Gonzales
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plant physiology     Volume:  59     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  1977 Jun 
Date Detail:
Created Date:  2010-06-29     Completed Date:  2010-06-29     Revised Date:  2010-09-14    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1082-4     Citation Subset:  -    
Affiliation:
Department of Horticulture, University of Illinois, Urbana, Illinois 61801.
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