| Isolation of uridine 5'-pyrophosphate glucuronic Acid pyrophosphorylase and its assay using p-pyrophosphate. | |
| | |
MedLine Citation:
|
PMID: 16659998 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
|
A procedure was devised to detect and assay uridine 5'-pyrophosphate (UDP)-glucuronic acid pyrophosphorylase in plant extracts. Substrates are UDP-glucuronic acid and (32)P-pyrophosphate, and the (32)P-uridine 5'-triphosphate produced is selectively adsorbed to charcoal. The charcoal adsorption procedure is a modification of that used to determine (32)P-adenosine 5'-triphosphate produced by adenosine 5'-pyrophosphate glucose pyrophosphorylase, and the modification greatly improves the retention of uridine 5'-triphosphate. |
| | |
Authors:
|
D B Dickinson; D Hyman; J W Gonzales |
Related Documents
:
|
10760258 - Peptide nucleic acids rather than rna may have been the first genetic molecule. 3718488 - The molecular structure of a rapidly formed oligomeric adenosine tetraphosphate derivat... 7479738 - Evidence for incorporation of intact dietary pyrimidine (but not purine) nucleosides in... 11098938 - Increased diversity within the hla-b*07 group: identification of the two novel alleles ... 1607018 - Anion permeation limits the uncoupling activity of fatty acids in mitochondria. 14730148 - Specific incorporation of l-glutamine into volicitin in the regurgitant of spodoptera l... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Plant physiology Volume: 59 ISSN: 0032-0889 ISO Abbreviation: Plant Physiol. Publication Date: 1977 Jun |
Date Detail:
|
Created Date: 2010-06-29 Completed Date: 2010-06-29 Revised Date: 2010-09-14 |
Medline Journal Info:
|
Nlm Unique ID: 0401224 Medline TA: Plant Physiol Country: United States |
Other Details:
|
Languages: eng Pagination: 1082-4 Citation Subset: - |
Affiliation:
|
Department of Horticulture, University of Illinois, Urbana, Illinois 61801. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Continuous, Automated Acetylene Reduction Assays Using Intact Plants.
Next Document: Zinc, Iron, and Chlorophyll Metabolism in Zinc-toxic Corn.